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Production of 1,2-epoxyalkanes from 1-alkenes by Nocardia corallina B-276

  • Keizo Furuhashi
  • Akira Taoka
  • Seiichi Uchida
  • Isao Karube
  • Shuichi Suzuki
Industrial Microbiology

Summary

A gaseous hydrocarbon-assimilating microorganism, Nocardia corallina, grew on 1-alkenes (C3, C4 and C13 to C18) and produced corresponding 1,2-epoxyalkanes. One of the products, 1,2-epoxytetradecane, had a positive rotation [α] D 20 =+8.6°. The concentration of propyleneoxide reached 0.6 g/l after 5 days of cultivation. Accumulation of 1,2-epoxytetradecane continued till the end of cultivation when the pH was not controlled, though production stopped in a 24 h cultivation when the pH was controlled at an optimum pH for growth. Propylene-grown cells produced 1,2-epoxytetradecane from 1-tetradecene and degraded tetradecane. In the presence of chloramphenicol, propylene-grown cells did not degraded tetradecane though 1,2-epoxytetradecane was produced from 1-tetradecene. A spontaneous mutant, which neither grew on propylene nor produced propyleneoxide, was isolated. This strain grew on tetradecane and 1-tetradecene but produced only a trace amount of 1,2-epoxytetradecane from 1-tetradecene.

Keywords

Propylene Chloramphenicol Trace Amount Spontaneous Mutant Propyleneoxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Keizo Furuhashi
    • 1
  • Akira Taoka
    • 1
  • Seiichi Uchida
    • 1
  • Isao Karube
    • 2
  • Shuichi Suzuki
    • 2
  1. 1.Bio Research Center Co.Toda City, SaitamaJapan
  2. 2.Research Laboratory of Resources UtilizationTokyo Institute of TechnologyYokohamaJapan

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