Histochemistry

, Volume 50, Issue 4, pp 313–318 | Cite as

A comparison between the one-step and the two-step copper thiocholine procedure for the cytochemical localization of cholinesterases

  • Biba Klinar
  • Miro Brzin
Article
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Summary

A comparison between the one-step and the two-step copper thiocholine procedure for the subcellular localization of cholinesterases was under-taken. The results indicate that only under experimental conditions with short incubation times and precise control of the conversion into sulphide is the localization of the primary precipitate and that of the secondary precipitate identical. It was concluded that the conversion of the primary precipitate into Cu-sulphide is not necessary and can lead to artefacts.

Keywords

Public Health Copper Sulphide Incubation Time Subcellular Localization 
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References

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Biba Klinar
    • 1
  • Miro Brzin
    • 1
  1. 1.Institute of Pathological Physiology, Faculty of MedicineUniversity of LjubljanaLjubljanaYugoslavia

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