Biochemical Genetics

, Volume 23, Issue 7–8, pp 585–590 | Cite as

Enzymatic activity of atypical oriental types of aldehyde dehydrogenases

  • Akira Yoshida
  • Vibha Dave


Catalytic activity of the atypical Oriental-type aldehyde dehydrogenase-2 (ALDH2) was considered to be null or severely diminished. Recently it was suggested that the atypical ALDH22retained about 30% of the specific activity of the usual ALDH21. We reexamined the problem by two-dimensional crossed immunoelectrophoresis. The usual Caucasian livers exhibited two distinctive precipitin peaks, one corresponding to the cytosolic ALDH1 and the other corresponding to the usual mitochondrial ALDH21, in both protein stain and enzyme activity stain. In contrast, the atypical Oriental livers exhibited two precipitin peaks in protein stain, but only one peak, corresponding to ALDH1, in enzyme activity stain. These results support the original notion that the atypical ALDH22is enzymatically inactive or far less active than the usual enzyme, refuting the idea of the atypical ALDH22with substantial enzyme activity.

Key words

aldehyde dehydrogenase atypical aldehyde dehydrogenase enzyme activity of mutant anzyme 


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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Akira Yoshida
    • 1
  • Vibha Dave
    • 1
  1. 1.Department of Biochemical GeneticsBeckman Research Institute of the City of HopeDuarte

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