Dendrotoxin from the venom of the green mamba, Dendroaspis angusticeps

A neurotoxin that enhances acetylcholine release at neuromuscular junctions
  • A. L. Harvey
  • E. Karlsson
Article

DOI: 10.1007/BF00502565

Cite this article as:
Harvey, A.L. & Karlsson, E. Naunyn-Schmiedeberg's Arch. Pharmacol. (1980) 312: 1. doi:10.1007/BF00502565

Summary

The venom of the green mamba, Dendroaspis angusticeps has previously been shown to produce neuromuscular facilitation by increasing acetylcholine release. After gel filtration and ion-exchange chromatography of the whole venom, a basic polypeptide with facilitatory actions was isolated. This polypeptide, named dendrotoxin, has 59 amino acid residues, probably with only 3 disulphide bonds and a blocked N-terminus.

When injected into conscious mice, dendrotoxin made the mice hypersensitive to external stimuli and subsequently produced respiratory paralysis. When tested on the isolated chick biventer cervicis nerve-muscle preparation, concentrations of dendrotoxin of 0.5 μg/ml (7×10−8 M) and greater, increased responses to indirect stimulation by 200–250%, without any increase in responses to submaximal concentrations of exogeneous acetylcholine, carbachol, KCl or direct stimulation. The augmentation was slow to develop, not reversed by washing, and could last several hours before slowly waning. Dendrotoxin did not produce spontaneous twitching or contractures.

It is concluded that dendrotoxin is not an anticholinesterase, does not affect receptor sensitivity or muscle contractility, but produces twitch augmentation by increasing the amount of acetylcholine released by nerve stimulation. Thus, dendrotoxin appears to represent a snake venom neurotoxin with unusual chemical and pharmacological properties.

Key words

Snake venoms Dendroaspis angusticeps Neurotoxins Neuromuscular facilitation Acetylcholine release 

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • A. L. Harvey
    • 1
  • E. Karlsson
    • 2
  1. 1.Department of Physiology and PharmacologyUniversity of StrathclydeGlasgowUK
  2. 2.Institute of BiochemistryUniversity of UppsalaUppsalaSweden
  3. 3.School of Physiology and PharmacologyUniversity of New South WalesKensingtonAustralia

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