The preparation of brush border membranes from rat kidney using an aqueous two-phase polymer system
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Summary
An aqueous two-phase polymer system (dextran — polyethylene glycol) has been employed to prepare a brush border membrane fraction from rat kidney. The procedure is rapid, simple and can be performed without the use of an ultracentrifuge. Brush borders isolated with this method are well preserved and of reasonable purity. The marker enzymes maltase and 5′-nucleotidase were enriched between 7 and 15 fold.
Key words
Brush Border Membrane Two-Phase System Maltase Alkaline PhosphatasePreview
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References
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