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A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass

  • G. Braunegg
  • B. Sonnleitner
  • R. M. Lafferty
Biotechnology and Bioengineering

Summary

The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-β-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l. Only 4 h is required from sampling from the fermenter till completion of the PHB determination.

Keywords

Biomass Chromatography Microbial Biomass Chromatographic Method Excellent Reproducibility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • G. Braunegg
    • 1
  • B. Sonnleitner
    • 1
  • R. M. Lafferty
    • 1
  1. 1.Institut für Biochemische Technologie und Lebensmittelchemie der Technischen Universität GrazGrazAustria

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