Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 331, Issue 4, pp 351–354 | Cite as

Opioid activities of human β-casomorphins

  • Gertrud Koch
  • Klaus Wiedemann
  • Hansjörg Teschemacher


Opioid activities of human β-casomorphin-4,-5,-7 and -8 and, for comparison, of the corresponding bovine β-casomorphins were studied in the guinea-pig ileum preparation. Binding parameters, i.e. K d -values and binding site concentrations, for the interaction of human and bovine β-casomorphins with opioid receptors in rat brain homogenates were determined in inhibition experiments, using [3H]-(d-Ala2, MePhe4, Gly-ol5)enkephalin, [3H]-(d-Ala2, d-Leu5)enkephalin and [3H]ethylketazocin as μ-, δ- and κ-opioid receptor ligands. Analysis of binding data was performed using a non-linear curve fitting program. All β-casomorphins examined displayed opioid activity. The affinity was highest for μ-receptors, less so for δ-receptors and lowest for κ-receptors. It is suggested that human β-casomorphins might play a role as “food hormones”.

Key words

Human β-casein Opioid peptides Opioid receptor ligands Human β-casomorphins Computerized binding data analysis 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Gertrud Koch
    • 1
  • Klaus Wiedemann
    • 1
  • Hansjörg Teschemacher
    • 1
  1. 1.Rudolf-Buchheim-Institut für Pharmakologie der Justus-Liebig-Universität GießenGießenFederal Republic of Germany

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