Micromethod for the quantitative determination of succinic acid in the fermentation media

  • H. Sočič
  • V. Gaberc-Porekar
Industrial Microbiology


A rapid and accurate method is described for the determination of succinic acid allowing direct application of the fermentation broth filtrate to TLC plate. Subsequent chromatographic separation on silica gel thin-layer and detection of succinic acid by a copper salt reagent, permits quantitative densitometric evaluation of succinic acid in the concentration range from 10 to 40 μg. The quantitative analyses are reproducible and the assay has a coefficient of variation of 3.2%.


Copper Fermentation Quantitative Analysis Quantitative Determination Chromatographic Separation 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • H. Sočič
    • 1
  • V. Gaberc-Porekar
    • 1
  1. 1.Chemical Institute ‘Boris Kidrič’ and ‘LEK’Pharmaceutical and Chemical FactoryLjubljanaYugoslavia

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