Advertisement

Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 310, Issue 1, pp 93–96 | Cite as

Extracellular 5-hydroxytryptamine inhibits 5-hydroxytryptamine release from rat brain cortex slices

  • M. Göthert
  • G. Weinheimer
Short Communication

Summary

Rat brain cortex slices preincubated with 3H-5-hydroxytryptamine were superfused with physiological salt solution and stimulated electrically, or they were superfused with Ca2+-free solution containing 25 mM K+ and stimulated by introduction of 1.3 mM CaCl2 for 2 min.

After blockade of neuronal 5-hydroxytryptamine (5-HT) uptake with clomipramine or paroxetine, the 3H overflow evoked by both methods of stimulation was decreased by unlabelled 5-HT and increased by methiothepin. The inhibition caused by 5-HT was antagonized by simultaneous administration of methiothepin. The inhibition by 5-HT of Ca2+-induced overflow was also observed in the presence of tetrodotoxin.

These results suggest than 5-HT regulates its own release from central serotoninergic neurones by activating presynaptic 5-HT autoreceptors, thus decreasing the availability of Ca2+ for stimulus-release coupling.

Key words

Presynaptic receptors 5-Hydroxytryptamine Serotoninergic neurones Calcium ions 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aghajanian, G.K., Haigler, H.J., Bennet, J.L.: Amine receptors in CNS: III 5-Hydroxytryptamine in brain. In: Handbook of Psychopharmacology, Vol. 6 (Iversen, L.L., Iversen, S.D., Snyder, S.H., eds.) pp. 63–96. New York: Plenum 1975Google Scholar
  2. Arbilla, S., Kamal, L., Langer, S.Z.: Presynatpic GABA autoreceptors on GABAergic nerve endings of the rat substantia nigra. Eur. J. Pharmacol. 57, 211–217 (1979)Google Scholar
  3. Beaudet, A., Descarries, L.: The monoamine innervation of rat cerebral cortex: synaptic and nonsynaptic axon terminals. Neuroscience 3, 851–860 (1978)Google Scholar
  4. Bourgoin, S., Artaud, F., Enjialbert, A., Héry, F., Glowinski, J., Hamon, M.: Acute changes in central serotonin metabolism induced by the blockade or stimulation of serotoninergic receptors during ontogenesis in the rat. J. Pharmacol. exp. Ther. 202, 519–531 (1977)Google Scholar
  5. Chase, T.N., Katz, R.I., Kopin, I.J.: Release of 3H-serotonin from brain slices. J. Neurochem. 16, 607–615 (1969)Google Scholar
  6. Cerrito, F., Raiteri, M.: Serotonin release is modulated by presynaptic autoreceptors. Eur. J. Pharmacol. 57, 427–430 (1979)Google Scholar
  7. Farnebo, L.-O., Hamberger, B.: Drug-induced changes in the release of 3H-monoamines from field stimulated rat brain slices. Acta physiol. scand., Suppl. 371, 35–44 (1971)Google Scholar
  8. Farnebo, L.-O., Hamberger, B.: Regulation of [3H] 5-hydroxytryptamine release from rat brain slices. J. Pharm. Pharmac. 26, 642–644 (1974)Google Scholar
  9. Göthert, M.: Ca2+-induced noradrenaline release from central noradrenergic neurons promoted by high K+ concentration or ionophore A 23187. Naunyn-Schmiedeberg's Arch. Pharmacol. 307, 29–37 (1979a)Google Scholar
  10. Göthert, M.: Inhibition of 5-hydroxytryptamine release from rat cerebral cortex slices by extracellular 5-hydroxytryptamine. Naunyn-Schmiedeberg's Arch. Pharmacol. 308, R 42 (1979b)Google Scholar
  11. Hamon, M., Bourgoin, S., Jagger, J., Glowinski, J.: Effects of LSD on synthesis and release of 5-HT in rat brain slices. Brain Res. 69, 265–280 (1974)Google Scholar
  12. Kuhar, M.J.: Neurotransmitter uptake: A tool in identifying neurotransmitter-specific pathways. Life Sci. 13, 1623–1634 (1973)Google Scholar
  13. Petersen, E.N., Olsson, S.-O., Squires, R.F.: Effects of 5-HT uptake inhibitors on the pressor response to 5-HT in the pithed rat. The significance of the 5-HT blocking property. Eur. J. Pharmacol. 43, 209–215 (1977)Google Scholar
  14. Reimann, W., Zumstein, A., Jackisch, R., Starke, K., Hertting, G.: Effect of extracellular dopamine on the release of dopamine in the rabbit caudate nucleus: evidence for a dopaminergic feedback inhibition. Naunyn-Schmiedeberg's Arhc. Pharmacol. 306, 53–60 (1979)Google Scholar
  15. Shaskan, E.G., Snyder, S.H.: Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J. Pharmacol. exp. Ther. 175, 404–418 (1970)Google Scholar
  16. Taube, H.D., Starke, K., Borowski, E.: Presynaptic receptor systems on the noradrenergic neurones of rat brain. Naunyn-Schmiedeberg's Arch. Pharmacol. 299, 123–141 (1977)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • M. Göthert
    • 1
  • G. Weinheimer
    • 1
  1. 1.Pharmakologisches Institut der Universität EssenEssen 1Federal Republic of Germany

Personalised recommendations