Advertisement

Continuous production of L-alanine from fumarate in a two-stage membrane reactor

  • Almuth-Sigrun Jandel
  • Helmut Hustedt
  • Christian Wandrey
Biotechnology

Summary

L-alanine was produced continuously from fumaric acid by means of soluble aspartase and L-aspartate-β-decarboxylase. The two reaction steps were carried out in two membrane reactors in series at different pH and temperature. The retention of the soluble enzymes within the reactor vessels was achieved by means of ultrafiltration membranes.

Keywords

Enzyme Fumarate Reactor Vessel Reaction Step Fumaric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chibata I, Kakimoto T, Kato J, Shibatani T, Nishimura N (1967) Cristalline aspartic β-decarboxylase of Pseudomonas dacunhae. Biochem Biophys Res Commun 26:662–672Google Scholar
  2. Kakimoto T, Kato J, Shibatani T, Nishimura N, Chibata I (1969) Cristalline L-aspartat β-decarboxylase of Pseudomonas dacunhae, I. cristallization and some physicochemical properties. J Biol Chem 244:353–358Google Scholar
  3. Kula MR (1979) Extraction and Purification of Enzymes. In: Wingard LB Jr, Katchalski-Katzir E, Goldstein L (eds) Applied Biochemistry and Bioengineering. Academic Press, New York 2:71–95Google Scholar
  4. Michaelis AS (1968) Ultrafiltration. Prog Sep Purif 1:297–334Google Scholar
  5. Sato T, Mori T, Tosa T, Chibata I, Furui M, Yamashita K, Sumi A (1975) Engineering analysis of continuous production of L-aspartic acid by immobilized escherichia-coli-cells in fixed beds. Biotechnol Bioeng 17:1797–1809Google Scholar
  6. Sato T, Nishida Y, Tosa T, Chibata I (1979) Immobilization of Escheria coli cells containing aspartase activity with k-carrageenan, enzymatic properties and application for L-aspartic acid production. Biochem Biophys Acta 570:179–186Google Scholar
  7. Suzuki S, Yamaguchi J, Tokushige M (1973) Studies on aspartase, 1. purification and molecular properties of aspartase from Escherichia coli. Biochem Biophys Acta 321:369–381Google Scholar
  8. Tate SS, Meister A (1969) Regulation of the activity of L-aspartate β-decarboxylase by a novel allosteric mechanism. Biochem 4:1660–1668Google Scholar
  9. Tate SS, Meister A (1970) Regulation and subunit structure of aspartate β-decarboxylase. Studies on the enzyme from Alcaligenis faecalis and Pseudomonas dacunhae. Biochem 13:2626–2632Google Scholar
  10. Tate SS, Meister A (1971) L-aspartate-β-decarboxylase; structure, catalytic activities, and allosteric regulation. Adv Enzymol Rela Areas Mol Biol 35:503–543Google Scholar
  11. Wandrey C, Flaschel E (1979) Process Development and Economical Aspects in Enzyme Engineering. Acylase L-Methionine System. In: Ghose TK, Fiechter A, Blakebrough N (eds) Adv Biochem Eng 12:147–218Google Scholar
  12. Wichmann R (1981) Kontinuierliche enzymatische Synthese mit Coenzym-Regeneration. Jül-Spez 119:1–166Google Scholar
  13. Yamamoto K, Tosa T, Chibata I (1980) Continuous production of L-alanine using Pseudomonas dacunhae immobilized with carrageenan. Biotechnol Bioeng 22:2045–2054Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Almuth-Sigrun Jandel
    • 1
  • Helmut Hustedt
    • 2
  • Christian Wandrey
    • 1
  1. 1.Kernforschungsanlage JülichInstitut für BiotechnologieJülichFederal Republic of Germany
  2. 2.Gesellschaft für Biotechnologische ForschungBraunschweigFederal Republic of Germany

Personalised recommendations