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Veterinary Research Communications

, Volume 15, Issue 1, pp 7–16 | Cite as

Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge

  • A. W. Hill
Bacteriology

Abstract

Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge. Vaccine A, a heat-killed Re mutant of strain K12, (UB 1574), was administered as a single parenteral and local dose to 5 cows with 3 control animals and Vaccine B, a heat-killed mutant of O111:B4 (J5) was administered as three parenteral doses into 5 cows with 5 control animals. Following intramammary challenge with a smooth wild-type strain (P4), an acute, severe clinical mastitis developed in all 14 quarters (9 vaccine A and 5 vaccine B) of the vaccinated animals which was indistinguishable from that in the 11 quarters of the control animals. Following vaccine B there was an elevation in serum IgG1 and IgG2 antibody to the common core antigen of endotoxin which, in contrast to the control animals, showed a further increase after intramammary infection.

Keywords

cattle Escherichia coli mastitis protection vaccination 

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References

  1. Bramley, A.J., 1985. The control of coliform mastitis. Proceedings of the 24th Annual Meeting of the National Mastitis Council, Las Vegas, pp. 4–17Google Scholar
  2. Bramley, A.J. and Neave, F.K., 1975. Studies in the control of coliform mastitis in dairy cows. British Veterinary Journal, 131, 160–169Google Scholar
  3. Chang, C.C., Winter, A.J. and Norcross, N.L., 1981. Immune response in the bovine mammary gland after intestinal, local and systemic immunization. Infection and Immunity, 31, 650–659Google Scholar
  4. Gaffin, S.L. and Lachmann, E., 1984. The use of antilipopolysaccharide (anti-LPS) antibodies in the management of septic shock. South African Medical Journal, 65, 158–161Google Scholar
  5. Gonzalez, R.N., Cullor, J.S., Jasper, D.E., Farver, T.B., Bushnell, R.B. and Oliver, M.N., 1989. Prevention of clinical coliform mastitis in dairy cows by a mutant Escherichia coli vaccine. Canadian Journal of Veterinary Research, 53, 301–305Google Scholar
  6. Hill, A.W., 1981. Factors influencing the outcome of Escherichia coli mastitis in the dairy cow. Research in Veterinary Science, 31, 107–112Google Scholar
  7. Hill, A.W., Shears, A.L. and Hibbitt, K.G., 1978. The elimination of serum resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Research in Veterinary Science, 25, 89–93Google Scholar
  8. Lisowski, J., Janusz, M., Tyran, B., Morawiecki, A., Golab, S. and Bialkowska, H., 1975. Immunoglobulins of colostrum. IV. Comparative studies of bovine serum and colostral IgG1 and IgG2. Immunochemistry, 12, 167–172Google Scholar
  9. Lohuis, J.A.C.M., Verheijden, J.H.M., Burvenich, C. and van Miert, A.S.J.P.A.M., 1988. Pathophysiological effects of endotoxins in ruminants. 1. Changes in body temperature and reticulo-rumen motility and the effect of repeated administration. The Veterinary Quarterly, 10, 109–116Google Scholar
  10. McCutchan, J.A., Zeigler, E.J. and Braude, A.I., 1979. Treatment of Gram-negative bacteraemia with antisera to core glycolipid. II Controlled trial with antisera in patients with bacteraemia. European Journal of Cancer, 15, 77–80Google Scholar
  11. Miles, A.A., Misra, S.S. and Irwin, J.O., 1938. The estimation of the bactericidal power of the blood. Journal of Hygiene (Cambridge), 38, 732–749Google Scholar
  12. Newby, T.J. and Bourne, F.J., 1977. The nature of the local immune system of the bovine mammary gland. Journal of Immunology, 118, 461–465Google Scholar
  13. Sheldrake, R.F., Husband, A.J., Watson, D.L. and Cripps, A.W., 1984. Selective transport of serum derived IgA into mucosal secretions. Journal of Immunology, 132, 363–367Google Scholar
  14. Tyler, J.W., Cullor, J.S., Osburn, B.I., Bushnell, R.B. and Fenwick, B.W., 1988. Relationship between serologic recognition of Escherichia coli O111:B5 (J5) and clinical coliform mastitis in cattle. American Journal of Veterinary Research, 49, 1950–1954Google Scholar
  15. Weigt, U., 1983. Clinical aspects of coliform mastitis in the bovine. Veterinary Research Communications, 7, 253–257Google Scholar
  16. Williams, M.R. and Hill, A.W., 1982. A role for IgM in the in vitro opsonisation of Staphylococcus aureus and Escherichia coli by bovine polymorphonuclear leucocytes. Research in Veterinary Science, 33, 47–53Google Scholar
  17. Zeigler, E.J., McCutchan, J.A., Fierer, J., Glauser, M.P., Sadoff, J.C., Douglas, H. and Braude, A.I., 1982. Treatment of Gram-negative bacteraemia and shock with human antisera to a mutant Escherichia coli. New England Journal of Medicine, 307, 1225–1230Google Scholar

Copyright information

© Kluwer Academic Publishers bv 1991

Authors and Affiliations

  • A. W. Hill
    • 1
  1. 1.Institute for Animal HealthNewburyUK

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