Histochemistry

, Volume 38, Issue 4, pp 297–305 | Cite as

New studies on the neural crest origin of the avian ultimobranchial glandular cells—Interspecific combinations and cytochemical characterization of C cells based on the uptake of biogenic amine precursors

  • N. Le Douarin
  • J. Fontaine
  • C. Le Lièvre
Article

Summary

Chick and quail ultimobranchial glandular cells are able to take up l-Dopa from the blood since the 11th day of incubation. They synthesize dopamine and show a faint greenish fluorescence which strongly contrasts during all the embryonic development with the bright fluorescence of carotid body cells. Isotopic and isochronic grafts of neural rhombencephalic primordium from 6 to 10-somite quail embryos are implanted into chick. The the carotid body and the ultimobranchial gland of the host are invaded by quail neurectodermic cells which have respectively the same cytochemical properties than the normal quail UB and CB glandular cells regarding their ability to take up l-Dopa and their subsequent fluorogenic amine content. Quail cells can be identified from host cells by their nuclear characteristics. The present work confirms earlier findings of neural crest origin of UBG cells using the quail-chick marker system associated with electron microscopic characteristics of calcitonin cells.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Almqvist, S., Malmqvist, E., Owman, C., Ritzén, M., Sundler, F., Swedin, G.: Dopamine synthesis and storage, calcium lowering activity and thyroidal properties of chicken ultimobranchial cells. Gen. comp. Endocr. 17, 512–525 (1971)Google Scholar
  2. Corrodi, H., Jonsson, G.: The formaldehyde fluorescence method for the histochemical demonstration of biogenic amines. A review on the methodology. J. Histochem. Cytochem. 15, 65–78 (1967)Google Scholar
  3. Falck, B.: Observations on the possibilities of the cellular localization of monoamines by a fluorescence method. Acta physiol. scand. 56, Suppl. 197, 1–25 (1962)Google Scholar
  4. Falck, B., Owman, C.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund. no. 7, 1–23 (1965)Google Scholar
  5. Fontaine, J.: Contribution à l'étude du développement du corps carotidien et du corps ultimobranchial des oiseaux. Le contenu en monoamines et la capacité de capter la L-Dopa des cellules glomiques et des cellules à calcitonine au cours de la vie embryonnaire. Arch. Anat. micr. morph. exp. 62, 89–100 (1973)Google Scholar
  6. Hachmeister, U., Kracht, J., Kruse, H., Lenke, M.: Lokalisation von C-Zellen im Ultimobranchial-Körper des Haushuhns. Naturwissenschaften 54, 619 (1967)Google Scholar
  7. Le Douarin, N.: Particularités du noyau interphasique chez la Caille japonaise (Coturnix coturnix japonica). Utilisation de ces particularités comme «marquage biologique» dans les recherches sur les interactions tissulaires et les migrations cellulaires au cours de l'ontogenèse. Bull. biol. Franc. Belg. 103, 435–452 (1969)Google Scholar
  8. Le Douarin, N.: Caractéristiques ultrastructurales du noyau interphasique chez la Caille et chez le Poulet et utilisation des cellules de Caille comme «marqueurs biologiques» en Embryologie expérimentale. Ann. Embryol. Morph. 4, 125–135 (1971)Google Scholar
  9. Le Douarin, N.: A biological cell labelling technique and its use in experimental embryology. Develop. Biol. 30, 217–222 (1973)Google Scholar
  10. Le Douarin, N., Le Lièvre, C.: Démonstration de l'origine neurale des cellules à calcitonine du corps ultimobranchial chez l'embryon de poulet. C. R. Acad. Sci. (Paris) 270, 2857–2860 (1970)Google Scholar
  11. Le Douarin, N., Le Lièvre, C.: Sur l'origine des cellules à calcitonine du corps ultimobranchial de l'embryon d'oiseau. C. R. Ass. Anat. 152, 558–568 (1971)Google Scholar
  12. Le Douarin, N., Le Lièvre, C.: Demonstration of the neural origin of the ultimobranchial body glandular cells in the avian embryo. Third. Int. Symp. Endocrinol.-July 1971. LondonGoogle Scholar
  13. Le Douarin, N., Le Lièvre, C., Fontaine, F.: Recherches expérimentales sur l'origine embryologique du corps carotidien chez les oiseaux. C. R. Acad. Sci. (Paris) 275, 583–586 (1972)Google Scholar
  14. Le Douarin, N., Teillet, M. A.: Sur quelques aspects de la migration des cellules neurales chez l'embryon de poulet étudiés par la méthode des greffes hétérospécifiques du tube nerveux. C. R. Soc. Biol. (Paris) 164, 390–397 (1970)Google Scholar
  15. Le Lièvre, C., Le Douarin, N.: Neural crest mesenchymal derivatives in the pharyngeal region (in press)Google Scholar
  16. Malmqvist, E., Ericson, L. E., Almqvist, S., Ekholm, R.: Granulated cells uptake of amine precursors and calcium lowering activity in the ultimobranchial body of the domestic fowl. Ultrastruct. Res. 23, 547–461 (1968)Google Scholar
  17. Mayor, H. D., Hampton, J. C., Rosario, B.: A simple method for removing the resin from epoxy-embedded tissue. J. biophys. biochem. Cytol. 9, 909–910 (1961)Google Scholar
  18. Pearse, A. G. E.: 5-hydroxytryptophan uptake by dog thyroid C cells and its possible significance in polypeptide hormone production. Nature (Lond.) 211, 598 (1966)Google Scholar
  19. Pearse, A. G. E.: The cytochemistry and ultrastructure of polypeptide hormone producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)Google Scholar
  20. Pearse, A. G. E., Polak, J. M.: Cytochemical evidence for the neural crest origin of mammalian ultimobranchial C. cells. Histochemie 27, 96–102 (1971)Google Scholar
  21. Pearse, A. G. E., Polak, J. M., Rost, F. W. D., Fontaine, J., Le Lièvre, C., Le Douarin, N.: Demonstration of the neural crest origin of Type I (APUD) cells in the avian carotid body, using a cytochemical marker system. Histochemie 34, 191–203 (1973)Google Scholar
  22. Stoeckel, M. E., Porte, A.: Etude ultrastructurale des corps ultimobranchiaux du poulet. I. Aspect normal et développement embryonnaire. Z. Zellforsch. 94, 495–512 (1969)Google Scholar
  23. Weston, J. A.: The migration and differentiation of neural crest cells. Advanc. Morph. 8, 41–114 (1970)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • N. Le Douarin
    • 1
  • J. Fontaine
    • 1
  • C. Le Lièvre
    • 1
  1. 1.Laboratory of EmbryologyNantesFrance

Personalised recommendations