Virchows Archiv A

, Volume 390, Issue 3, pp 339–345 | Cite as

Morphology of foci of altered hepatocytes and naturally-occurring hepatocellular tumors in F344 rats

  • J. M. Ward
Original Articles


The morphology of liver tumors of F344 rats used as controls in carcinogenesis bioassays were studied. Foci of cellular alteration composed of hepatocytes with basophilic cytoplasm were found commonly in F344 rats, 2 years of age. Eosinophilic and vacuolated foci were considerably less common. The morphology of 67 nodular hepatic lesions indicated that 54 were neoplastic nodules and 13 hepatocellular carcinomas. The majority of these tumors were composed of basophilic hepatocytes. Some of the carcinomas appeared to arise within neoplastic nodules. No tumors metastasized.

Key words

Liver neoplasms Hepatocellular carcinoma F344 rat 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adler JH, Roderig H, Ungar H (1976) Neoplastic liver nodules of unknown causes in a colony of Sand rats (Psammomys obesus). Israel J Med Science 12:1212–1215Google Scholar
  2. Bannasch P (1978) Sequential cellular alterations during hepatocarcinogenesis. In: Butler W, Newberne PM (eds) Rat hepatic neoplasia. MIT Press, Cambridge p 58–93Google Scholar
  3. Goodman DG, Ward JM, Squire RA, Chu KC, Linhart MS (1979) Neoplastic and nonneoplastic lesions in aging F344 rats. Toxicol Appl Pharmacol 48:237–248Google Scholar
  4. Hirota N, Williams GM (1979) Persistence and growth of rat liver neoplastic nodules following cessation of carcinogen exposure. J Natl Cancer Inst 63:1257–1265Google Scholar
  5. Hoover K, Ward JM, Stinson SF (1980) Histopathological differences between liver tumors in untreated mice and mice fed diets containing nitrofen. J Natl Cancer Inst, 65:937–948Google Scholar
  6. Institute of Laboratory Animal Resources (1980) Histologic typing of liver tumors of the rat. J Natl Cancer Inst 64:177–206Google Scholar
  7. Pitot HC (1977) The natural history of neoplasia. Am J Pathol 89:401–412Google Scholar
  8. Pollard M, Luckert PH (1979) Spontaneous liver tumors in aged germ free Wistar rats. Lab An Science 29:74–77Google Scholar
  9. Reznik G, Ward JM (1979) Carcinogenicity of the hair dye component 2-nitro-p-phenylenediamine (2-HPPN): induction of eosinophilic hepatocellular neoplasms in female B6C3F1 mice. Fd Cosmet Toxicol 17:493–500Google Scholar
  10. Squire RA, Levitt MH (1975) Report of a workshop on classification of specific hepatocellular lesions in rats. Cancer Res 35:3214–3215Google Scholar
  11. Ward JM (1980) Morphology of hepatocellular neoplasms in B6C3F1 mice. Cancer Lett 9:319–325Google Scholar
  12. Ward JM, Bernal E, Buratto B, Goodman DG, Standberg JD, Schueler R (1979) Histopathology of neoplastic and nonneoplastic hepatic lesions in mice fed diets containing tetrachlorvinphos. J Natl Cancer Inst 63:111–118Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • J. M. Ward
    • 1
  1. 1.Tumor Pathology Branch, Carcinogenesis Testing Program, National Cancer Institute, National Toxicology ProgramNational Institutes of HealthBethesdaUSA

Personalised recommendations