, Volume 61, Issue 2, pp 103–109 | Cite as

Immunofluorescent and biochemical studies on tyrosine hydroxylase and dopamine-β-hydroxylase of the bullfrog sciatic nerves

  • I. Nagatsu
  • Y. Kondo
  • S. Inagaki
  • H. Kojima
  • T. Nagatsu


Immunofluorescence specific for tyrosine hydroxylase (TH) or dopamine-β-hydroxylase (DBH) was accentuated in both proximal and distal segments of the sciatic nerve after ligation. Estimations of the enzyme activities confirmed the above results. Mean axoplasmic flow rates of TH and DBH in bullfrog sciatic nerve were found to be 8 and 123 mm/day, respectively. They were decreased by colchicine or by cold temperatures (4° C).


Public Health Enzyme Activity Tyrosine Colchicine Sciatic Nerve 
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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • I. Nagatsu
    • 1
  • Y. Kondo
    • 1
  • S. Inagaki
    • 1
  • H. Kojima
    • 2
  • T. Nagatsu
    • 3
  1. 1.Department of Anatomy, School of MedicineFujita-Gakuen UniversityToyoake, AichiJapan
  2. 2.Institute of Brain DiseasesKurume University School of MedicineKurumeJapan
  3. 3.Laboratory of Cell Physiology, Department of Life Chemistry, Graduate School at NagatsutaTokyo Institute of TechnologyYokohamaJapan

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