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Histochemistry

, Volume 45, Issue 2, pp 101–113 | Cite as

Etude cytochimique des hémocytes des crustacés décapodes brachyoures

  • Adrien G. Bauchau
  • Marie-Bernadette De Brouwer
  • Elisabeth Passelecq-Gerin
  • Jacques C. Mengeot
Article

Résumé

Les hémocytes de deux espèces de crabes, Eriocheir sinensis et Carcinus maenas, ont été soumis à différentes réactions cytochimiques en vue de préciser la nature des contenus granulaires, soit par des colorations spécifiques, soit par des digestions enzymatiques. Cette étude a été faite tant en microscopie optique qu'électronique.

Les granules contiennent essentiellement des protéines basiques, riches en arginine et en ponts disulfures mais pauvres en tyrosine. Elles portent en outre des chaînes glucidiques non glycogéniques. Des dépôts de glycogène d'importance variable sont distribués dans le cytoplasme de tous les types cellulaires mais n'ont jamais été observés dans les granules euxmêmes. Les lipides sont peu abondants. La masse granulaire se compose essentiellement de mucopolysaccharides neutres quoique de petites quantités de mucosubstances acides aient pu être mises en évidence quelquefois.

Cytochemical observations of hemocytes in decapod brachyuran crustacea

Summary

Hemocytes from two species of crabs, Eriocheir sinensis and Carcinus maenas, have been submitted to different cytochemical reactions in order to bring out the nature of granules' content, either by specific coloration or by enzymatic digestion. Light as well as electron microscopy has been used.

The granules appeared to be made essentially of basic proteins, rich in arginin and disulfide bridges but poor in tyrosin. These proteins are linked with nonglycogenic carbohydrates. Glycogen deposits of varied size are restricted to the cytoplasm in all the different cell-types but have never been detected inside the granules themselves. Lipids are poorly represented. The bulk of the granule material is made of neutral mucopolysaccharides although minute amounts of acid mucosubstances could be found in some instances.

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Références

  1. Arvy, L.: Contribution à l'étude du sang et de la leucopoièse chez Pachygrapsus marmoratus Fabr. Ann. Sci. Nat. Zool. 11è Sér. 14, 1–12 (1952)Google Scholar
  2. Bauchau, A. G., De Brouwer, M. B.: Ultrastructure des hémocytes d'Eriocheir sinensis, Crustacé Décapode Brachyoure. J. Microsc. 15, 171–179 (1972)Google Scholar
  3. Bauchau, A. G., De Brouwer, M. B.: Etude ultrastructurale de la coagulation de l'hémolymphe chez les crustacés. J. Microsc. 19, 37–46 (1974)Google Scholar
  4. Bauchau, A. G., Plaquet, J. C.: Variation du nombre des hémocytes chez les Crustacés Brachyoures. Crustaceana 24, 215–223 (1973)Google Scholar
  5. Bazin, F., Demeusy, N.: Processus de cicatrisation consécutif à l'autotomie d'un péreiopode chez le crabe Carcinus maenas (L.) C. R. Acad. Sci. (Paris) 274D, 2603–2605 (1972)Google Scholar
  6. Beaulaton, J.: Etude ultrastructurale et cytochimique des glandes prothoraciques des vers à soie aux quatrième et cinquième âges larvaires. I. La tunica propria et ses relations avec les fibres conjonctives et les hémocytes. J. Ultrastruct. Res. 23, 474–498 (1968)Google Scholar
  7. Dumont, J. N., Anderson, E., Winner, G.: Some cytologic characteristics of the hemocytes of Limulus during clotting. J. Morph. 119, 181–208 (1966)Google Scholar
  8. Fahrenbach, W. H.: The cyanoblast. Hemocyanin formation in Limulus polyphemus. J. Cell Biol. 44, 445–453 (1970)Google Scholar
  9. Gabe, M. Techniques histologiques. Paris: Masson 1968Google Scholar
  10. Ganser, P., Jolles, G.: Histochimie normale et pathologique. Paris: Gauthier-Villars 1969–1970Google Scholar
  11. George, W. C., Nichols, T.: A study of the blood of some crustacea. J. Morph. 83, 425–440 (1948)Google Scholar
  12. Ghiretti-Magaldi, A., Milanesi, C., Salvato, C.: Identification of hemocyanin in the cyanocytes of Carcinus maenas. Experientia (Basel) 29, 1265–1267 (1973)Google Scholar
  13. Hardin, J. H., Spicer, S. S.: Ultrastructural localisation of dialysed iron reactive mucosubstance on rabbit heterophils, basophils and eosinophils. J. Cell Biol. 48, 368–386 (1971)Google Scholar
  14. Hearing, V., Vernick, S. H.: Fine structure of the blood cells of the lobster, Homarus americanus. Chesapeake Sci. 8, 170–186 (1967)Google Scholar
  15. Horn, R. G., Spicer, S. S.: Sulfated mucopolysaccharide and basic protein in certain granules of rabbit leukocytes. Lab. Invest. 13, 1–15 (1964)Google Scholar
  16. Johnston, M. A., Davies, P. S.: Carbohydrates of the hepatopancreas and blood tissues of Carcinus. Comp. Biochem. Physiol. 41 B 433–443 (1972)Google Scholar
  17. Johnston, M. A., Davies, P. S., Elder, H. Y.: Possible hepatic function for crustacean blood cells. Nature (Lond.) 230, 471–472 (1971)Google Scholar
  18. Johnston, M. A., Elder, H. Y., Davies, P. S.: Cytology of Carcinus haemocytes and their function in carbohydrate metabolism. Comp. Biochem. Physiol. 46 A, 569–582 (1973)Google Scholar
  19. Lai-Fook, J.: The structure of the haemocytes of Calpodes ethlius (Lepidoptera) J. Morph. 139, 79–104 (1973)Google Scholar
  20. Lavallard, R., Campiglia, S.: Contribution à l'hématologie de Peripatus acacioi Marcus et Marcus (Onycophore). I. Structure et ultrastructure des hémocytes. Ann. Sci. Nat. Zool. 12è Sér. 17, 67–92 (1975)Google Scholar
  21. Lochhead, J. H. and Lochhead, M. S.: Studies on the blood and related tissues in Artemia (Crustacea Anostraca). J. Morph. 68, 593–632 (1941)Google Scholar
  22. Marschall, K. J.: Bau und Funktion der Blutzellen des Mehlkäfers Tenebrio molitor L. Z. Morph. ökol. Tiere 58, 182–246 (1966)Google Scholar
  23. Monneron, A., Bernhad, W.: Action de certains enzymes sur des tissus inclus en Epon. J. Microsc. 5, 697–714 (1966)Google Scholar
  24. Neuwirth, M.: The structure of hemocytes of Galleria mellonella (Lepidoptera) J. Morph. 139, 105–124 (1973)Google Scholar
  25. Pearse, A. G. E.: Histochemistry, 3d edit. London: Churchill 1968Google Scholar
  26. Rambourg, A.: Localisation ultrastructurale et nature du matériel coloré au niveau de la surface cellulaire par le mélange chromique-phosphotungstique. J. Microsc. 8, 325–342 (1969)Google Scholar
  27. Scharrer, B.: Cytophysiological features of hemocytes in cockroaches. Z. Zellforsch. 129, 301–319 (1972)Google Scholar
  28. Spicer, S. S.: Diamine methods for differenciating mucosubstances histochemically. J. Histochem. Cytochem. 13, 211–234 (1965)Google Scholar
  29. Spicer, S. S., Jarrels, M. H.: Histochemical reaction of an aromatic diamine with acid groups and periodate engendered aldehydes in mucopolysaccharides. J. Histochem. Cytochem. 9, 368–379 (1961)Google Scholar
  30. Spicer, S. S., Leppi, T. J., Stoward, P. J.: Suggestions for a histochemical terminology of carbohydrate-rich tissue compounds. J. Histochem. Cytochem. 13, 599–603 (1965)Google Scholar
  31. Stang-Voss, C.: Zur Ultrastruktur der Blutzellen wirbelloser Tiere. V. Über die Hämocyten von Astacus astacus (L.) (Crustacea). Z. Zellforsch. 122, 68–75 (1971)Google Scholar
  32. Thiery, J. P.: Mise en evidence des polysaccharides sur coupes fines en microscopie électronique. J. Microsc. 6, 987–1018 (1967)Google Scholar
  33. Wood, P. J., Visentin, L. P.: Histological and histochemical observations of the hemolymph cells in the crayfish, Orconectes virilis. J. Morph. 123, 559–568 (1967)Google Scholar
  34. Wood, P. J., Podlewski, J., Shenk, T. E.: Cytochemical observations of the hemolymph cells during coagulation in the crayfish, Orconectes virilis. J. Morph. 134, 479–488 (1971)Google Scholar
  35. Zeya, H. I., Spitznagel, J. K.: Arginin rich-proteins of polymorphonuclear leukocyte lysosomes. J. exp. Med. 127, 927–941 (1968)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Adrien G. Bauchau
    • 1
  • Marie-Bernadette De Brouwer
    • 1
  • Elisabeth Passelecq-Gerin
    • 1
  • Jacques C. Mengeot
    • 1
  1. 1.Laboratoire de Biologie AnimaleFacultés Universitaires Notre-Dame de la PaixNamurBelgique

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