Histochemistry

, Volume 74, Issue 1, pp 153–156

The influence of tissue section thickness on the study of collagen by the Picrosirius-polarization method

  • L. C. U. Junqueira
  • G. S. Montes
  • E. M. Sanchez
Short Communication

DOI: 10.1007/BF00495061

Cite this article as:
Junqueira, L.C.U., Montes, G.S. & Sanchez, E.M. Histochemistry (1982) 74: 153. doi:10.1007/BF00495061

Summary

The influence of tissue section thickness on the color and intensity of birefringence displayed by collagen in tissue sections studied by means of the Picrosirius-polarization method, is reported in this paper. When dermal collagen sections of different thicknesses (ranging from 0.25 to 11 μm) were studied by this method, it became evident that not only did the intensity of birefringence increase proportionally to tissue section thickness, as was to be expected, but also a gradual shift in color from green through yellow to red could be observed as tissue section thickness increased. The limitations of the Picrosirius-polarization method for the localization of collagen types I, II, and III in routinely used histological slides is discussed, showing that this method is useful for the study of the distribution of the different types of interstitial collagen in normal adult vertebrate organs.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • L. C. U. Junqueira
    • 1
  • G. S. Montes
    • 1
  • E. M. Sanchez
    • 1
  1. 1.Laboratories for Cell Biology and Experimental OncologyUniversity of São Paulo School of MedicineSão PauloBrazil

Personalised recommendations