Histochemistry

, Volume 73, Issue 1, pp 49–56 | Cite as

Cytochemical detection of proteases in synovial fluid of arthritic joints

  • M. -L. Johannes
  • C. Klessen
Article
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Summary

A cytochemical method can be used to detect neutral proteases in polymorphonuclear leukocytes (PMN cells) in synovial fluid smears obtained from arthritic joints. Using this method, incubation of fixed smears in NaCl-borate buffer causes release of neutral PMN proteases, the enzymatic activity of which is documented in the degradation of exudate protein in ring-shaped areas centered around the neutrophils (halo effect). Such halo formation can also be detected around certain large mononuclear cells, as well as around very small particles — which may represent thrombocytes. Application of trypsin inhibitors and various antirheumatic agents causes concentration-dependent inhibition of halo formation.

Keywords

Enzymatic Activity Trypsin Mononuclear Cell Small Particle Synovial Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • M. -L. Johannes
    • 1
  • C. Klessen
    • 1
  1. 1.Anatomisches Institut der Universität TübingenTübingen 1Federal Republic of Germany

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