Histochemistry

, Volume 80, Issue 1, pp 31–38 | Cite as

Pancreatic duct glands. II. Lectin binding affinities of ductular epithelium, ductular glands, and Brunner glands

  • S. Geleff
  • P. Böck
Article

Summary

Secretory epithelia and ductular glands were studied in main pancreatic ducts of guinea pig, rabbit, rat, and man. Brunner glands were studied for comparison. Semithin sections from Epon-embedded tissues were etched with sodium methylate and incubated with horseradish peroxidase-conjugated lectins. Columnar cells in the epithelium of pancreatic ducts are endowed with well-developed microvillar borders. These apical regions strongly stain with Lotus A-, wheat germ-, and Ricinus I-lectins. Basolateral plasma membranes bind Ricinus I-, Ulex europaeus I-, and wheat germ-lectins. Cytosomes in the supranuclear regions of epithelial cells are interpreted as secretory granules. These droplets are marked by wheat germ-lectin and to a lesser degree by Ricinus I- and Ulex europaeus I-lectins. Ductular glands of the main pancreatic ducts contain secretions that bind Helix-, wheat germ-, and Ulex europaeus I-lectins. Their apical and basolateral cell membranes deeply stain with wheat germ- and Ulex europaeus I-lectins. Secretions of Brunner glands bind Ricinus I-, Ulex europaeus I-, Helix-, and wheat germ lectins. Their apical and basolateral cell membranes stain with Ricinus I- and wheat germ-lectins.-Species differences in lectin-binding affinities of complex carbohydrates were observed and are described.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Belanger LF (1963) Comparison between different histochemical and histophysical techniques as applied to mucous secreting cells. Ann NY Acad Sci 106:364–378Google Scholar
  2. Berger C, Pizzolato P (1975) The staining of Brunner's gland and other neutral mucins by carmin, haematoxylin and orcein in alkaline solutions. Stain Technol 42:389–409Google Scholar
  3. Bernhard W, Avrameas S (1971) Ultrastructural visualization of cellular carbohydrate components by means of Concanavalin A. Exp Cell Res 64:232–236Google Scholar
  4. Böck P (1978) Pancreatic duct glands. I. Staining reaction of acid glycoprotein secret. Acta Histochem (Jena) 61:118–126Google Scholar
  5. Gemmel RT, Heath T (1973) Structure and function of the biliary and pancreatic tracts of the sheep. J Anat 115:221–236Google Scholar
  6. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302Google Scholar
  7. Graumann W (1953) Zur Standardisierung des Schiffschen Reagens. Z wiss Mikrosk 61:225–226Google Scholar
  8. Kaissling B (1973) Histologische und histochemische Untersuchungen an semidünnen Schnitten. Gegenbauers morphol Jahrb 119:1–13Google Scholar
  9. Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414Google Scholar
  10. McMinn RMH, Kugler JH (1961) The glands of the bile and pancreatic duct: autoradiographic and histochemical studies. J Anat 95:1–11Google Scholar
  11. Nicolson GL (1974) The interaction of lectins with animal cell surfaces. Int Rev Cytol 39:89–190Google Scholar
  12. Obuoforibo AA (1975) Mucosubstances in Brunner's gland of the mouse. J Anat 119:287–294Google Scholar
  13. Oduor-Okelo D (1976) Histochemistry of the duodenal glands of the cat and horse. Acta Anat (Basel) 94:449–456Google Scholar
  14. Poddar S, Jacob S (1979) Mucosubstance histochemistry of Brunner's gland, pyloric glands and duodenal goblet cells in the ferret. Histochemistry 65:67–81Google Scholar
  15. Sheahan DG, Jervis HR (1976) Comparative histochemistry of gastroinestinal mucosubstances. Am J Anat 146:103–132Google Scholar
  16. Spicer SS (1963) Histochemical differentiation of mammalian mucopolysaccharide. Ann NY Acad Sci 106:379–388Google Scholar
  17. Spicer SS, Meyer DB (1960) Histochemical differentiation of acid mucopolysaccharide by means of combined aldehyde fuchsinalcian blue staining. Am J Clin Pathol 33:453–460Google Scholar
  18. Thiéry J-P, Rambourg A (1974) Cytochimie de polysaccharides. J Microscopie 21:225–232Google Scholar
  19. Treasure T (1978) The ducts of Brunner's glands. J Anat 127:299–304Google Scholar
  20. Trump BF, Smuckler EA, Benditt EP (1961) A method for staining epoxy sections for light microscopy. J Ultrastruct Res 5:343–348Google Scholar
  21. Zimmermann KW (1927) Die Speicheldrüsen der Mundhöhle und die Bauchspeicheldrüsen. In: Möllendorff W v (ed) Handbuch der mikroskopischen Anatomie des Menschen, vol V/1. Springer, Berlin, pp 61–244Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Geleff
    • 1
  • P. Böck
    • 1
  1. 1.Department of Micromorphology and Electron MicroscopyUniversity of ViennaViennaAustria

Personalised recommendations