, Volume 75, Issue 1, pp 53–65 | Cite as

No classical type IIB fibres in dog skeletal muscle

  • D. H. Snow
  • R. Billeter
  • F. Mascarello
  • E. Carpene
  • A. Rowlerson
  • E. Jenny


To analyse the fibre type composition of adult dog skeletal muscle, enzyme histochemistry, immunohistochemistry for type I, IIA and IIB myosins, and peptide mapping of myosin heavy chains isolated from typed single fibres were combined. Subdivision of type II fibres into two main classes according to the activity of the m-ATPase after acidic and alkaline preincubation proved to be rather difficult and was only consistently achieved after a very careful adjustment of the systems used. One of these sub-classes of type II fibres stained more strongly for m-ATPase activity after acidic and alkaline preincubation, was oxidative-glycolytic and showed a strong reaction with an anti-type IIA myosin. The other one, however, although unreactive with anti-IIA myosin, was also oxidative-glycolytic, and only showed a faint reaction with an anti-type IIB myosin. Peptide mapping of the myosin heavy chains of typed single fibres revealed two populations of heavy chains among the type II fibre group. Thus, in dog muscle, we are confronted with the presence of two main classes of type II fibres, both oxidative-glycolytic, but differing in the structure of their myosin heavy chains. In contrast to some reports in the literature, no classical type IIB fibres could be detected.


Peptide Heavy Chain Fibre Type Myosin Heavy Chain Single Fibre 
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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • D. H. Snow
    • 1
  • R. Billeter
    • 2
  • F. Mascarello
    • 3
  • E. Carpene
    • 4
  • A. Rowlerson
    • 2
  • E. Jenny
    • 2
  1. 1.Department of Veterinary PharmacologyUniversity of Glasgow, Veterinary SchoolGlasgowUK
  2. 2.Institute of Pharmacology and BiochemistryUniversity of ZürichZürichSwitzerland
  3. 3.Instituto di Anatomia degli Animali Domestici con Istologia col EmbriologiaUniversità di MilanoMilanoItaly
  4. 4.Istituto di Biochimica, Facoltà di Medicina VeterinariaUniversità di BolognaBolognaItaly

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