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Histochemistry

, Volume 54, Issue 3, pp 237–250 | Cite as

Sudan Black B: Chemical structure and histochemistry of the blue main components

  • U. Pfüller
  • H. Franz
  • A. Preiß
Article

Summary

Sudan Black B contains two blue main components, SSB-I and SSB-II. Their chemical structures were determinated by the aid of twodimensional thin-layer chromatography, column chromatography, absorption, IR, mass, H1-NMR, and C13-NMR spectroscopy and were proved by alternate synthesis. SSB-I has been found to be 2,3-dihydro-2,2-dimethyl-4-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. For SSB-II was confirmed the known structure 2,3-dihydro-2,2-dimethyl-6-[(4-phenylazo-l-naphthalenyl)-azo]-lH-perimidine. Relations of chemical structure of SSB-I and SSB-II to their staining properties are discussed.

Keywords

Public Health Column Chromatography Staining Property Alternate Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • U. Pfüller
    • 1
    • 2
  • H. Franz
    • 1
    • 2
  • A. Preiß
    • 1
    • 2
  1. 1.State Institute for Immune Preparates and Nutrition MediaBerlinGerman Democratic Republic
  2. 2.VEB Chemiekombinat BitterfeldBitterfeldGerman Democratic Republic

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