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Histochemistry

, Volume 78, Issue 1, pp 121–124 | Cite as

Myoglobin concentration in single type I and type II muscle fibres in man

  • E. Jansson
  • C. Sylvén
Article

Summary

Histochemical visualization of myoglobin in a benzidine peroxidase reaction suggests that human skeletal muscle fibres are differentiated into fibres having either high or a low myoglobin content. In the present study myoglobin was quantified in single human muscle fibres after being classified as either type I (“slow-twitch”) or type II (“fast twitch”). Samples were obtained from m. quadriceps femoris in 4 healthy untrained male subjects using the needle biopsy technique. After freeze-drying, individual fibres were dissected out and classified as either type I or type II by a myofibrillar ATPase stain. Myoglobin analyses were performed on these single fibres by a radioimmunoassay. The myoglobin concentrations were found to be significantly higher in type I than in type II muscle fibres in all 4 subjects and were, on the average, of the same magnitude as found in larger (mixed muscle) samples. The myoglobin concentration ratio between type I and type II fibres ranged from 1.4 to 1.7.

Keywords

Muscle Fibre Single Fibre Human Skeletal Muscle Human Muscle Skeletal Muscle Fibre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • E. Jansson
    • 1
  • C. Sylvén
    • 1
  1. 1.Departments of Clinical Physiology, Clinical Chemistry and Internal MedicineKarolinska SjukhusetStockholmSweden

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