The ultrastructural demonstration of mitochondrial ATPase activity by the ATP-dependent accumulation of Ca++
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Summary
A new method was developed for the ultrastructural demonstration of mitochondrial ATPase activity based on the ATP dependent mitochondrial accumulation of Ca++. Reaction product labels the inner mitochondrial compartment in the form of electron dense precipitate granules. Reaction could be blocked with 10 mM oligomycin.
Keywords
Public Health ATPase Activity Oligomycin Mitochondrial ATPase Dense Precipitate
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