Biochemical Genetics

, Volume 21, Issue 11–12, pp 1057–1070 | Cite as

The tissue source and cellular control of the apparent size of androgen binding protein (Abp), a mouse salivary protein whose electrophoretic mobility is under the control of Sex-limited saliva pattern (Ssp)

  • Stephen R. Dlouhy
  • Robert C. Karn


Expression of the dominant allele, SspSat the Sex-limited saliva pattern locus in mice results in an alteration of the electrophoretic mobility of a mouse salivary protein from the F (fast) to the S (slow) type [Karn, R. C., et al. (1980). Genetics94:s52; Karn, R. C., et al. (1982). Biochem. Genet.20:493]. We now demonstrate that the protein affected binds androgen and has a basic heterodimeric structure with the subunits connected by disulfide bridging. It is produced in the submaxillary gland where the alteration takes place. The change in electrophoretic mobility from F to S appears to be primarily the result of an increase in the molecular weight of the larger subunit since the isoelectric point changes very little. We discuss the possible causes of the increase in molecular weight.

Key words

mouse salivary protein heterodimeric structure androgen binding testicular feminization two-dimensional electrophoresis 


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Copyright information

© Plenum Publishing Corporation 1983

Authors and Affiliations

  • Stephen R. Dlouhy
    • 1
  • Robert C. Karn
    • 1
  1. 1.Department of Medical GeneticsIndiana University School of MedicineIndianapolis

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