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Biochemical Genetics

, Volume 12, Issue 2, pp 121–145 | Cite as

Genetic variation and population ecology of some southeast asian frogs of the genera Bufo and Rana

  • Robert F. Inger
  • Harold K. Voris
  • Helen H. Voris
Article

Abstract

The relationship between population structure and amount of genetic variation within populations has been investigated in seven species of anurans from Southeast Asia. Two populations each of Bufo asper, B. parvus, B. melanostictus, Rana blythi, and R. erythraea and one population each of R. hosei and R. laticeps were studied; all except B. melanostictus and R. erythraea are forest species. Information on distributional characteristics such as general habitat, form of the adult range, the relationships of nonbreeding range to breeding site, the tendency to aggregate at the breeding site, and the size of the breeding aggregation was used to outline major aspects of the breeding structure of each species and predict relative amounts of genic variation. Genetic variation was estimated from measures of protein polymorphism as detected through gel electrophoresis. The assays used were liver EST, FUM, GDH, LAP, LDH, MDH, and total protein; muscle EST, GOT, MDH, and total protein; plasma EST and total protein. The pattern of variation of the populations of Bufo species agreed with predictions based on population structure, with B. asper the least polymorphic, B. melanostictus the most polymorphic, and B. parvus intermediate. Interspecific differences were not as great in Rana as in Bufo. Although R. erythraea was more variable than R. blythi in terms of mean number of morphs per zone (as predicted from distributional characteristics), R. blythi possessed polymorphism at more zones. R. hosei was the most polymorphic Rana studied, but distributional data are insufficient to explain why this was the case.

Key words

electrophoresis ecology Asian frogs genetic variation 

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References

  1. Alcala, A. C. (1955). Observations on the life history and ecology of Rana erythraea Schlegel, on Negros Island, Philippines. Silliman J. 2175.Google Scholar
  2. Boulenger, G. A. (1912). Reptilia and Batrachia ... in a Vertebrate Fauna of the Malaya Peninsula, Taylor & Francis, London.Google Scholar
  3. Church, G. (1960). Annual and lunar periodicity in the sexual cycle of the Javanese toad, Bufo melanostictus Schneider. Zoologica (Sci. Cont. N. Y. Zool. Soc. Pt. 4) 44181.Google Scholar
  4. Dessauer, H. C. (1966). Multiple localizations of specific proteins on starch gel electropherograms. Rutgers Univ. Serol. Mus. Bull. 361.Google Scholar
  5. Dessauer, H. C., and Nevo E. (1969). Geographic variation of blood and liver proteins in cricket frogs. Biochem. Genet. 3171.Google Scholar
  6. Gillespie, J. H., and Kojima, K. (1968). The degree of polymorphisms in enzymes involved in energy production compared to that in nonspecific enzymes in two Drosophila ananassae populations. Proc. Natl. Acad. Sci. 61582.Google Scholar
  7. Hubby, J. L., and Lewontin, R. C. (1966). A molecular approach to the study of genic heterozygosity in natural populations. I. The number of alleles at different loci in Drosophila pseudoobscura. Genetics 54577.Google Scholar
  8. Hubby, J. L., and Throckmorton, L. H. (1965). Protein differences in Drosophila. II. Comparative species genetics and evolutionary problems. Genetics 52203.Google Scholar
  9. Hunter, R. L., and Strachan, D. S. (1961). Esterases of mouse blood. Ann. N.Y. Acad. Sci. 94(1243861.Google Scholar
  10. Inger, R. F. (1966). The systematics and zoogeography of the Amphibia of Borneo. Fieldiana Zool. 521.Google Scholar
  11. Inger, R. F. (1969). Organization of communities of frogs along small rain forest streams in Sarawak. J. Anim. Ecol. 38123.Google Scholar
  12. Inger, R. F., and Greenberg, B. (1963). The annual reproductive pattern of the frog Rana erythraea in Sarawak. Physiol. Zool. 36(121.Google Scholar
  13. Johnson, W. E., and Selanders, R. K. (1971). Protein variation and systematics in kangaroo rats (genus Dipodomys). Syst. Zool. 20377.Google Scholar
  14. Kimura, M., and Crow, J. F. (1964). The number of alleles that can be maintained in a finite population. Genetics 49725.Google Scholar
  15. Koehn, R., and Mitton, J. (1972). Population genetics of marine pelecypods. I. Ecological heterogeneity and evolutionary strategy at an enzyme locus. Am. Naturalist 106(94747.Google Scholar
  16. Lakovaara, S., Saura, A., and Falk, C. T. (1972). Genetic distance and evolutionary relationships in the Drosophila obscura group. Evolution 26(2177.Google Scholar
  17. Lewontin, R. C., and Hubby, J. L. (1966) A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54595.Google Scholar
  18. Menhinick, E. F. (1964). A comparison of some species diversity indices applied to samples of field insects. Ecology 45(4859.Google Scholar
  19. Nair, P. S., Brncic, D., and Kojima, K. (1971). II. Isozyme variations and evolutionary relationships in the mesophragmatica species group of Drosophila. Stud. Genet. (Univ. Texas Publ.) 6(710317.Google Scholar
  20. Petras, M. L. (1963). Genetic control of a serum esterase component in Mus musculus. Proc. Natl. Acad. Sci. 50112.Google Scholar
  21. Petras, M. L. (1967). Studies of natural populations of Mus. I. Biochemical polymorphisms and their bearing on breeding structure. Evolution 21259.Google Scholar
  22. Prakash, S., Lewontin, R. C., and Hubby, J. L. (1969). A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central, marginal and isolated populations of Drosophila pseudoobscura. Genetics 61(4841.Google Scholar
  23. Ruddle, F. H. (1966). Kidney esterases of the mouse (Mus musculus): Electrophoretic analysis of inbred lines C57B/6J, RF/J and SJL/J. J. Histochem. Cytochem. 14(125.Google Scholar
  24. Selander, R. K., and Yang, S. Y. (1969). Protein polymorphism and genic heterozygosity in a wild population of the house mouse (Mus musculus). Genetics 63653.Google Scholar
  25. Selander, R. K., Yang, S. Y., Lewontin, R. C., and Johnson, W. E. (1970). Genetic variation in the horseshoe crab (Limulus polyphemus), a phylogenetic “relic.”. Evolution 24(2402.Google Scholar
  26. Shaw, C. R., and Koen, A. L. (1968). Starch gel zone electrophoresis of enzymes. In Smith, I. (ed.), Chromatographic and Electrophoretic Techniques, Vol. II, Interscience, New York.Google Scholar
  27. Shaw, C. R., and Prasad, R. (1970). Starch gel electrophoresis of enzymes—A compilation of recipes. Biochem. Genet. 4297.Google Scholar
  28. Siegel, S. (1956). Nonparametric Statistics for the Behavioral Sciences, McGraw-Hill, New York.Google Scholar
  29. Smith, M. A. (1917). A list of the batrachians at present known to inhabitat Siam. J. Nat. Hist. Soc. Siam 2226.Google Scholar
  30. Wright, S. (1951). The genetical structure of populations. Ann. Eugen. 15323.Google Scholar

Copyright information

© Plenum Publishing Corporation 1974

Authors and Affiliations

  • Robert F. Inger
    • 1
  • Harold K. Voris
    • 1
  • Helen H. Voris
    • 1
  1. 1.Field Museum of Natural HistoryChicago

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