Biochemical Genetics

, Volume 12, Issue 4, pp 257–269 | Cite as

Genome size and the proportion of repeated nucleotide sequence DNA in plants

  • R. B. Flavell
  • M. D. Bennett
  • J. B. Smith
  • D. B. Smith
Article

Abstract

The reannealing kinetics of denatured DNA fragments from 23 species of higher plants have been studied, using hydroxylapatite chromatography to distinguish reannealed from single-stranded DNA. The 2C nuclear DNA contents of the species varied between 1.7 and 98 pg. The proportions of DNA in species with a nuclear DNA mass above 5 pg that reannealed with the kinetics of sequences present in more than 100 copies were high (69–92% with a mean of 80±2.0%). For species with less than 4 pg of DNA, the mean proportion of repeated-sequence DNA was 62±2.9%. It is concluded that most of the variation in nuclear DNA mass in higher plant chromosomes can be accounted for by variation in repeated-sequence DNA. The consequences of altering the adapted DNA content of a species by the addition of families of repeated sequences are discussed in relation to the proportion of repeated-sequence DNA.

Key words

DNA repeated sequences plant DNA 

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Copyright information

© Plenum Publishing Corporation 1974

Authors and Affiliations

  • R. B. Flavell
    • 1
  • M. D. Bennett
    • 1
  • J. B. Smith
    • 1
  • D. B. Smith
    • 1
  1. 1.Department of CytogeneticsPlant Breeding InstituteTrumpington, CambridgeEngland

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