Biochemical Genetics

, Volume 4, Issue 2, pp 297–320 | Cite as

Starch gel electrophoresis of enzymes—A compilation of recipes

  • Charles R. Shaw
  • Rupi Prasad
Methods

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References

  1. Allen, S. L., Misch, M. S., and Morrison, B. M. (1963). Acid phosphatase of tetrahymena. J. Histochem. Cytochem. 11 706.Google Scholar
  2. Baptist, J. N., Shaw, C. R., and Mandel, M. (1969). Unpublished data.Google Scholar
  3. Beckman, L., and Johnson, F. M. (1964). Esterase variations in Drosophila melanogaster. Hereditas 51 212.Google Scholar
  4. Boyer, S. H. (1961). Alkaline phosphatase in human sera and placentae. Science 134 1002.Google Scholar
  5. Brewer, G. J. (1966). Personal communication.Google Scholar
  6. Dawson, M., Eppenberger, H. M., and Kaplan, N. O. (1965). Creatine kinase: evidence for a dimeric structure. Biochem. Biophys. Res. Commun. 21 346.Google Scholar
  7. Delorenzo, R. J., and Ruddle, F. H. (1969). Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem. Genet. 3 151.Google Scholar
  8. Der Kaloustian, V. M., Byrne, R., Young, W. J., and Childs, B. (1969). An electrophoretic method for detecting hypoxanthine-guanine phosphoribosyl transferase variants. Biochem. Genet. 3 299.Google Scholar
  9. Eaton, G. M., Brewer, G. J., and Tashian, R. E. (1966). Hexokinase isoenzyme patterns of human erythrocytes and leukocytes. National Meeting on Hematology, 1966 (abst.).Google Scholar
  10. Fildes, R. A., and Harris, H. (1966). Genetically determined variation of adenylate kinase in man. Nature 209 261.Google Scholar
  11. Fondo, E. Y., Jr., and Bartalos, M. (1969). Elecrophoretic separation of multiple bands with beta-glucuronidase activity in human sera. Biochem. Genet. 3 591.Google Scholar
  12. Fottrell, P. F., and O'Hora, A. (1969). Multiple forms of D (—)3-hydroxybutyrate dehydrogenase in Rhizobium. J. Gen. Microbiol. 57 287.Google Scholar
  13. Graham, R. C., Lundholm, U., and Karnovsky, M. J. (1965). Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole. J. Histochem. Cytochem. 13 150.Google Scholar
  14. Hanley, W. B., Boyer, S. H., and Naughton, M. A. (1966), Electrophoretic and functional heterogeneity of pepsinogen in several species. Nature 209 996.Google Scholar
  15. Henderson, N. S. (1965). Isozymes of isocitrate dehydrogenase: Subunit structure and intracellular location. J. Exp. Zool. 158 263.Google Scholar
  16. Hunter, R., and Markert, C. (1957). Histochemical demonstration of enzymes separated by zone electrophoresis in starch gel. Science 125 1294.Google Scholar
  17. Jolley, R. L., Jr., and Mason, H. S. (1965). Multiple forms of mushroom tyrosinase. J. Biol. Chem. 240 1489.Google Scholar
  18. Koen, A. L. (1969). Personal communication.Google Scholar
  19. Koen, A. L., and Shaw, C. R. (1966). Retinol and alcohol dehydrogenases in retina and liver. Biochim. Biophys. Acta 128 48.Google Scholar
  20. Lin, C., Schipmann, G., Kittrel, W. A., and Ohno, S. (1969). The predominance of heterozygotes found in wild goldfish of Lake Erie at the gene locus for sorbitol dehydrogenase. Biochem. Genet. 3 603.Google Scholar
  21. Lewis, W. H. P., and Harris, H. (1967). Human red cell peptidases. Nature 215 351.Google Scholar
  22. Migeon, B. R., Smith, S. W., and Leddy, C. L. (1969). The nature of thymidine kinase in the human-mouse hybrid cell. Biochem. Genet. 3 583.Google Scholar
  23. Pearse, A. G. E. (1961). Histochemistry. Little, Brown, Boston.Google Scholar
  24. Poulik, M. D., and Bearn, A. G. (1962). Heterogeneity of ceruloplasmin. Clin. Chim. Acta 7 374.Google Scholar
  25. Ressler, N., Olivero, E., Thompson, G. R., and Joseph, R. R. (1966). Investigation of ribonuclease isozymes by an electrophoretic ultra-violet method. Nature 210 695.Google Scholar
  26. Robinson, K. (1966). An examination of Corynebacterium spp. by gel electrophoresis. J. Appl. Bacteriol. 29 179.Google Scholar
  27. Schwartz, M. K., Nisselbaum, J. S., and Bodansky, O. (1963). Procedure for staining zones of activity of glutamic oxaloacetic transaminase following electrophoresis with starch gel. Am. J. Clin. Pathol. 40 103.Google Scholar
  28. Selander, R. K., and Yang, S. Y. (1969). Protein polymorphism and genic heterozygosity in a wild population of the house mouse (Mus musculus). Genetics (in press).Google Scholar
  29. Selander, R. K., Yang, S. Y., and Hunt, W. G. (1969). Polymorphism in esterases and hemoglobin in 3 wild populations of the house mouse (Mus musculus). In Studies in Genetics V, University of Texas Publication No. 6918, p. 271.Google Scholar
  30. Shaw, C. R., and Baptist, J. N. (1969). Unpublished data.Google Scholar
  31. Shaw, C. R., and Koen, A. L. (1964). Aspartate dehydrogenase activity of malate dehydrogenase. Biochim. Biophys. Acta 92 397.Google Scholar
  32. Shaw, C. R., and Koen, A. L. (1965). On the identity of “nothing dehydrogenase.” J. Histochem. Cytochem. 13 431.Google Scholar
  33. Shaw, C. R., and Koen, A. L. (1968a). Glucose 6-phosphate dehydrogenase and hexose 6-phosphate dehydrogenase of mammalian tissues. Ann. N. Y. Acad. Sci. 151 149.Google Scholar
  34. Shaw, C. R., and Koen, A. L. (1968b). Zone electrophoresis of enzymes. In Smith, I. (ed.), Chromatography and Electrophoresis, 2nd ed., Interscience Publishers, New York.Google Scholar
  35. Smith, E. E., and Rutenberg, A. M. (1966). Starch-gel electrophoresis of human enzymes which hydrolyze L-leucine-β-naphthylamide. Science 152 1256.Google Scholar
  36. Spencer, N., Hopkinson, D. A., and Harris, H. (1964). Phosphoglucomutase polymorphism in man. Nature 204 742.Google Scholar
  37. Stout, D. (1969). Unpublished data.Google Scholar
  38. Tashian, R. E. (1969). The esterases and carbonic anhydrases of human erythrocytes In Yunis, J. (ed.), Biochemical Method in Red Cell Genetics, Academic Press, New York, p. 307.Google Scholar
  39. Tsuyuki, H., and Wold, F. (1964) Enolase: Multiple forms in fish muscle. Science 146 535.Google Scholar
  40. Williams, R. A. D. (1964). Location of glyceraldehyde-3-phosphate dehydrogenase in starch gel. Nature 203 1070.Google Scholar

Copyright information

© Plenum Publishing Corporation 1970

Authors and Affiliations

  • Charles R. Shaw
    • 1
  • Rupi Prasad
    • 1
  1. 1.Department of BiologyThe University of Texas, M. D. Anderson Hospital and Tumor InstituteHouston

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