Biochemical Genetics

, Volume 19, Issue 11–12, pp 1101–1114 | Cite as

The genetics and biochemistry of urease in Ustilago violacea

  • Michael L. Baird
  • Edward D. Garber
Article

Abstract

Two complementing loci in different linkage groups of the basidiomycete Ustilago violacea are involved in urease activity: a structural one (ure-1) and a second inferred to involve a permease (ure-2) locus. Two types of complementing mutations occur in the structural locus: null activity (ure-1a) and obviously reduced activity (ure-1b). The ure-2 mutants lacked urease activity in vivo on the phenol red-urea test medium, but gave extracts with wild-type activity. Extracts from wild-type strains gave one site of urease activity after polyacrylamide gel electrophoresis. A number of ure-1b mutants and active revertants from ure-1a mutants yielded electrophoretically variant urease sites. The results are discussed in terms of enzyme polymorphism in haploid eukaryotes by one (missense) or two (null, then missense) mutations.

Key words

urease electrophoresis Ustilago violacea 

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • Michael L. Baird
    • 1
  • Edward D. Garber
    • 2
  1. 1.Department of Human Genetics and DevelopmentColumbia UniversityNew York
  2. 2.Department of BiologyUniversity of ChicagoChicago

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