Biochemical Genetics

, Volume 15, Issue 11–12, pp 1181–1191 | Cite as

Induction of the alcohol-metabolizing pathway in the nematode Panagrellus redivivus: Phenotypic effects

  • Frank Kriger
  • Dan Burke
  • M. R. Samoiloff
Article

Abstract

Panagrellus redivivus when placed in 7% ethanol or methanol becomes immobile. After 1 hr the animals resume normal swimming and will grow in alcohol. The ability to recover requires ADH activity and translation, but not transcription, as determined by inhibitor studies. Recovery decreases with longer-chain alcohols, with a greater recovery for branched rather than n-alcohols. Coincident with recovery is a threefold increase in alcohol dehydrogenase and aldehyde dehydrogenase activity. A model involving posttranscriptional control of the levels of these enzymes is presented.

Key words

nematode enzyme induction alcohol dehydrogenase aldehyde dehydrogenase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aueron, F., and Rothstein, M. (1974). Nematode biochemistry. XIII. Peroxisomes in the free-living nematode Turbatrix aceti. Comp. Biochem. Pjysiol. 49B:261.Google Scholar
  2. Brenner, S (1974). The genetics of Caenorhabditis elegans. Genetics 7771.Google Scholar
  3. Briscoe, D. A., Robertson, A., and Malpica, J. M. (1975). Dominance of ADH locus in adult Drosophila melanogaster to environmental alcohol. Nature 235148.Google Scholar
  4. David, J. R., Bocquet, C., Arens, M., and Fouillet, P. (1976). Biological roles of alcoholdehydrogenase in tolerance of Drosophila melanogaster to aliphatic alcohols: utilization of an ADH null mutant. Biochem. Genet. 14989.Google Scholar
  5. Elliot, J. I., and Knapp, J. A. (1975). Alcohol dehydrogenase from Drosophila melanogaster. In Wood, W. A. (ed.), Methods in Enzymology, Vol. 41, Academic Press, New York.Google Scholar
  6. Kriger, F., and Samoiloff, M. R. (1974). A rapid method for separating larvae from a mixed population of Panagrellus redivivus. J. Nematol. 6217.Google Scholar
  7. Leevy, C., and Zetterman, R. (1975). Malnutrition and alcoholism. In Rothchild, M. A., Oratz, M., and Schneiber, S. (eds.), Alcohol and Aldehyde Protein Biosynthesis, Pergamon Press, London, pp. 3–15.Google Scholar
  8. Morgan, P. (1975). Selection acting directly on an enzyme polymorphism. Heredity 34124.Google Scholar
  9. Nicholas, W. L. (1975). The Biology of Free Living Nematodes, Oxford University Press, Oxford.Google Scholar
  10. Oakeshott, J. G. (1976). Selection of the alcohol dehydrogenase in Drosophila melanogaster imposed by environmental alcohol. Genet. Res. 26265.Google Scholar
  11. Pasternak, J., and Samoiloff, M. R. (1970). The effects of growth inhibitors on post-embryonic development in the free-living nematode Panagrellus silusiae. Comp. Biochem. Physiol. 3327.Google Scholar
  12. Samoiloff, M. R., McNicholl, P., Cheng, R., and Balakanich, S. (1973). Regulation of nematode behaviour by physical means. Exp. Parasitol. 33253.Google Scholar
  13. Schwartz, D. (1971). Genetic control of alcohol dehydrogenase—A competition model for regulation of gene action. Genetics 67411.Google Scholar
  14. Schwartz, M., and Sofer, W. (1976). Alcohol dehydrogenase-negative mutants in Drosophila: Defects at the structural locus. Genetics 83125.Google Scholar
  15. Sin, W. C., and Pasternak, J. (1970). Number and DNA content of nuclei in the free-living nematode Panagrellus silusiae at each stage during post-embryonic development. Chromosoma 32191.Google Scholar
  16. Tottmar, S. O. C., Pettersson, H., and Kiessling, K. H. (1973). The subcellular distribution and properties of aldehyde dehydrogenase in rat liver. Biochem J. 135577.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Frank Kriger
    • 1
  • Dan Burke
    • 1
  • M. R. Samoiloff
    • 1
  1. 1.GANS, Department of ZoologyUniversity of ManitobaWinnipegCanada

Personalised recommendations