Biochemical Genetics

, Volume 16, Issue 1–2, pp 79–99 | Cite as

Genetic protein polymrophisms in human saliva: An interpretive review

  • Edwin A. Azen
Article
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Revised:

Abstract

The purpose of this review is to summarize recent progress in the field of genetic protein polymorphisms found in human saliva since 1972. Prior to 1972 most of the investigations were related to amylase. The genetics of salivary amylase will not be considered here, since it has recently been thoroughly reviewed elsewhere (Merritt and Karn, 1977). In this review, special attention will be devoted to the complex interrelationships of the proline-rich (Pr), double-band (Db), acidic protein (Pa), and peroxidase (SAPX) systems. The biochemically related Pr, Db, and Pa systems show distinctive genetic patterns, and there are associations between the phenotypes indicating linkage relationships. There is also evidence for probable interaction of products of the Pa and SAPX loci. Electrophoretic properties of these proteins can be defined in several gel systems, permitting an accurate definition of phenotypes. The usefulness and limitations of the different gel systems in the interpretation of these electrophoretic patterns will be illustrated. Allelic frequencies of the systems to be discussed are given in Table I. To aid comprehension, the systems will be discussed in logical rather than historical sequence.

Key words

acid phosphatase amylase carboxylesterase genetics glucose-6-phosphate dehydrogenase peroxidase polymorphism salivary proteins 
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References

  1. Armstrong, W. G. (1970). Amino acid composition of human parotid salivary proteins selectively adsorbed by hydroxy-apatite. Arch. Oral Biol. 151001.Google Scholar
  2. Armstrong, W. G. (1971). Characterization studies of the specific human salivary proteins adsorbed in vitro by hydroxy-apatite. Caries Res. 5215.Google Scholar
  3. Arneberg, P. (1974). Partial characterization of five glycoprotein fractions secreted by the human parotid glands. Arch. Oral Biol. 19921.Google Scholar
  4. Azen, E. A. (1972). Genetic polymorphism of basic proteins from parotid saliva. Science 176673.Google Scholar
  5. Azen, E. A. (1973). Properties of salivary basic proteins showing polymorphism. Biochem. Genet. 969.Google Scholar
  6. Azen, E. A. (1977). Salivary peroxidase (SAPX): Genetic modification and relationship to the proline-rich (Pr) and acidic (Pa) proteins. Biochem. Genet. 159.Google Scholar
  7. Azen, E. A., and Denniston, C. L. (1974). Genetic polymorphism of human salivary proline-rich proteins: Further genetic analysis. Biochem. Genet. 12109.Google Scholar
  8. Azen, E. A., and Oppenheim, F. G. (1973). Genetic polymorphism of proline-rich human salivary proteins. Science 1801067.Google Scholar
  9. Balakrishnan, C. R., and Ashton, G. C. (1974). Polymorphism of human salivary proteins. Am. J. Hum. Genet. 26145.Google Scholar
  10. Balekjian, A. Y., and Longton, R. W. (1973). Histones isolated from human parotid fluid. Biochem. Biophys. Res. Commun. 50676.Google Scholar
  11. Baum, B. J., Bird, J. L., Millar, D. B., and Longton, R. W. (1976) Studies on histidine rich polypeptides from human parotid saliva. Arch. Biochem. Biophys. 177427.Google Scholar
  12. Bennick, A. (1975). Chemical and physical characteristics of a phosphoprotein from human parotid saliva. Biochem. J. 145557.Google Scholar
  13. Bennick, A., and Connell, G. E. (1971). Purification and partial characterization of four proteins from human parotid saliva. Biochem. J. 123455.Google Scholar
  14. Bonner, W. M., and Laskey, R. A. (1974). A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 4683.Google Scholar
  15. Cutting, J. A., and Roth, T. F. (1973). Staining of phosphoproteins on acrylamide gel electropherograms. Anal. Biochem. 54386.Google Scholar
  16. Friedman, R. D., and Allushuski, R. (1975). An improved method for the detection of anionic polymorphic proteins in human saliva on polyacrylamide slab gels. Anal. Biochem. 65561.Google Scholar
  17. Friedman, R. D., and Karn, R. C. (1977). Immunological relationships and a genetic interpretation of major and minor acidic proteins in human parotid saliva. Biochem. Genet. 15549.Google Scholar
  18. Friedman, R. D., and Merritt, A. D. (1975). Partial purification and characterization of a polymorphic protein (Pa) in human parotid saliva. Am. J. Hum. Genet. 27304.Google Scholar
  19. Friedman, R. D., Merritt, A. D., and Rivas, M. L. (1975). Genetic studies of human acidic salivary protein (Pa). Am. J. Hum. Genet. 27292.Google Scholar
  20. Green, M. R., Pastewka, J. V., and Peacock, A. C. (1973). Differential staining of phosphoproteins on polyacrylamide gels with a cationic carbocyanine dye. Anal. Biochem. 5643.Google Scholar
  21. Hay, D. I. (1973). The interaction of human parotid salivary proteins with hydroxyapatite. Arch. Oral Biol. 181517.Google Scholar
  22. Hay, D. I. (1975). Fractionation of human parotid salivary proteins and the isolation of an histidine-rich acidic peptide which shows high affinity for hydroxyapatite surfaces. Arch. Oral Biol. 20553.Google Scholar
  23. Hay, D. I., and Oppenheim, F. G. (1974). The isolation from human parotid saliva of a further group of proline rich proteins. Arch. Oral Biol. 19627.Google Scholar
  24. Holbrook, I. B., and Molan, P. C. (1975). The identification of a peptide in human parotid saliva particularly active in enhancing the glycolytic activity of the salivary micro-organisms. Biochem. J. 149489.Google Scholar
  25. Laskey, R. A., and Mills, A. D. (1975) Quantitative film detection of 3H and 14C in poly-acrylamide gels by fluorography. Eur. J. Biochem. 56335.Google Scholar
  26. Levine, M., and Keller, P. J. (1977). The isolation of some basic proline-rich proteins from human parotid saliva. Arch. Oral Biol. 2237.Google Scholar
  27. Levine, M. J., Ellison, S. A., and Bahl, O. P. (1973). The isolation from human parotid saliva and partial characterization of the protein core of a major parotid glycoprotein. Arch. Oral Biol. 18827.Google Scholar
  28. Mandel, I. D., and Ellison, S. A. (1965). Organic components of human parotid and submaxillary saliva. Ann. N. Y. Acad. Sci. 131802.Google Scholar
  29. Merritt, A. D., and Karn, R. C. (1977). Human α-amylases. In Harris, H., and Hirschhorn, K. (ed.), Advances in Human Genetics, Vol. 8, Plenum, New York, p. 135.Google Scholar
  30. Merritt, A. D., Rivas, M. L., Bixler, D., and Newell, R. (1973). Salivary (Amy1) and pancreatic (Amy2) amylase: characterizations and genetic studies. Am. J. Hum. Genet. 25510.Google Scholar
  31. Nawrot, C. F., Campbell, D. J., Schroeder, J. K., and Van Valkenburg, M. (1976). Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate. Biochemistry 153445.Google Scholar
  32. Oppenheim, F. G., Hay, D. I., and Franzblau, C. (1971). Proline-rich proteins from human parotid saliva. 1. Isolation and partial characterization. Biochemistry 104233.Google Scholar
  33. Peters, E. H. (1974). Partial characterization of allelic variants of basic proteins from human parotid saliva. Ph.D. thesis, University of Wisconsin, Madison, Wis.Google Scholar
  34. Peters, E. H., and Azen, E. A. (1977). Isolation and partial characterization of human parotid basic proteins. Biochem. Genet. 15925.Google Scholar
  35. Peters, E. H., Goodfriend, T., and Azen, E. A. (1977). Human Pb, human post-Pb and non human primate Pb proteins: Immunological and biochemical relationships. Biochem. Genet. 15947.Google Scholar
  36. Slowey, R. F., Eidelman, S., and Klebanoff, S. J. (1968). Antibacterial activity of purified peroxidase from human parotid saliva. J. Bacteriol. 96575.Google Scholar
  37. Sung, M., and Smithies, O. (1969). Differential clution of histones from gel-trapped nuclei. Biopolymers 739.Google Scholar
  38. Tan, S. G. (1976). Human saliva esterases: Genetic studies. Hum. Hered. 26207.Google Scholar
  39. Tan, S. G., and Ashton, G. C. (1976a). An autotomal glucose-6-phosphate dehydrogenase (hexose-6-phosphate dehydrogenase) polymorphism in human saliva. Hum. Hered. 26113.Google Scholar
  40. Tan, S. G., and Ashton, G. C. (1976b). Saliva acid phosphatases: Genetic studies. Hum. Hered. 2681.Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Edwin A. Azen
    • 1
  1. 1.Department of MedicineUniversity of WisconsinMadison

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