Biochemical Genetics

, Volume 16, Issue 3–4, pp 271–286 | Cite as

Genetic and biochemical analysis of cycloheximide resistance in the fungus Podospora anserina

  • Marc Crouzet
  • Michel Perrot
  • Maria Nogueira
  • Joël Bégueret
Article
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Revised:

Abstract

Genetic analysis of cycloheximide-resistant mutants has shown that at least three genes control the resistance to cycloheximide in Podospora anserina and that the antibiotic resistance is recessive to sensitivity. In vitro and in vivo studies of protein synthesis indicated that for two mutants cycloheximide resistance is associated with the ribosomes. For one of these mutants, the elongation step in protein biosynthesis is insensitive to cycloheximide over a wide range of concentration. In this mutant the resistance to cycloheximide is a property of the 60S subunit.

Key words

cycloheximide resistance ribosomes Podospora anserina 
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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Marc Crouzet
    • 1
  • Michel Perrot
    • 1
  • Maria Nogueira
    • 1
  • Joël Bégueret
    • 1
  1. 1.Laboratorie de GénétiqueUniversité de Bordeaux IITalence-CedexFrance

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