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Quantitative determination of moniliformin in vegetable foods and feeds

Quantitative Bestimmung von Moniliformin in pflanzlichen Nahrungs- und Futtermitteln

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Zusammenfassung

Eine geeignete Analysenmethode zur Bestimmung von Moniliformin in Nahrungs- und Futtermitteln wird beschrieben. Das Toxin wurde aus verschimmeltem Mais, Reis, Roggen, Hafer, Weizen und Gerste extrahiert. Der Extrakt wurde anschließend dünnschicht-chromatographisch getrennt und Moniliformin mit N-Methylbenzthiazolon-2-hydrazon (MBTH), einem für den DC-Nachweis neuen Reagens, nachgewiesen. Das gebildete Moniliforminderivat konnte bei 518 nm in Remission bestimmt werden. Mit Hilfe einer Eichkurve wurden die erhaltenen Daten quantitativ ausgewertet. Ein linearer Zusammenhang zwischen Substanzmenge und Peakfläche besteht für den Bereich von 100–400 ng pro Fleck. Die Nachweisgrenze beträgt nach dieser Methode 75 ng/Fleck.

Summary

A suitable and simple method for the quantitative determination of moniliformin in vegetable foods and feeds is described. The mycotoxin was extracted by Soxhlet extraction with methanol from mouldy maize, rice, rye, oats, wheat and barley samples. Moniliformin was determined by thin-layer chromatography (TLC) using N-methylbenzthiazolon-2-hydrazone (MBTH) as a new derivatization reagent for this mycotoxin. The moniliformin derivative was assayed at 518 nm. Quantification could be performed after calibration. A linear relationship between mycotoxin amount and peak area was found from 100 to 400 ng/spot. The detection limit is 75 ng/spot.

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Authors and Affiliations

  1. Institut für Biochemie, Johannes-Gutenberg-Universität, Johann-Joachim-Becherweg 30, D-6500, Mainz, Federal Republic of Germany

    Christa Jansen & Klaus Dose

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  1. Christa Jansen
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  2. Klaus Dose
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Jansen, C., Dose, K. Quantitative determination of moniliformin in vegetable foods and feeds. Z. Anal. Chem. 319, 60–62 (1984). https://doi.org/10.1007/BF00476230

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  • DOI: https://doi.org/10.1007/BF00476230

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