The effect of furosemide on glucose oxidation of the cochlea and other tissues
Article
Received:
Accepted:
- 19 Downloads
Summary
We tested the hypothesis that furosemide interferes with energy generation in the cochlea, and determined its effect on CO2 formation from glucose and glyceroaldehyde-3-phosphate dehydrogenase (GAPDH) activity by examining biochemical and histochemical changes in the cochlea, the kidney, and the liver. We found that furosemide suppressed CO2 formation in vitro at relatively low concentrations in all tissues examined. GAPDH was inhibited as soon as 2 min after its administration (80 mg/kg, i.v.). Recovery of this enzyme activity was most rapid in the liver. We concluded that furosemide does interfere with energy generation in the cochlea, kidney, and liver as a result of its inhibition of GAPDH.
Key words
Furosemide 14CO2 formation 14C-glucosee Glyceroaldehyde-3-phosphate dehydrogenasePreview
Unable to display preview. Download preview PDF.
References
- 1.Case DB, Gurther SJ, Cannon (1973) Ethacrynate-induced depression of respiration in transport systems and kidney mitochondria. Am J Physiol 224:769–780Google Scholar
- 2.Eknoyan G (1974) Effect of diuretics on O2 consumption rate (Q O2) of renal cortical (C) and out medullary (OM) mitochondria of dogs. J Clin Invest 53:22AGoogle Scholar
- 3.Epstein RW (1972) The effects of ethacrynic acid on active transport of sugars and ions on other metabolic processes in rabbit kidney cortex. Biochim Biophys Acta 274:128–139Google Scholar
- 4.Goldschmidt D, Morelis R, Gaudenmer Y, Gautheron D (1970) Influence de l'ethacrynate sur la perméabilité des mitochondries de foie de rat aux cations K+ et Mg++. Bull Soc Chim Biol 52:523–529Google Scholar
- 5.Gordon EE (1968) Site of ethacrynic acid action on Ehrlich ascites tumor cells. Biochem Pharmacol 17:1237–1242Google Scholar
- 6.Gordon EE, de Hartog M (1971) Localization and characterization of the inhibitory action of ethacrynic acid on glycolysis. Biochem Pharmacol 17:1237–1242Google Scholar
- 7.Himmelhoch SR, Karnovsky MJ (1961) The histochemical demonstration of glyceroaldehyde-3-phosphate dehydrogenase activity. J Biophys Biochem Cytol 9:573–581Google Scholar
- 8.Kimura H, Kuriyama K (1975) A new microassay method for 1-glutamic acid decarboxylase activity. Jpn J Pharmacol 25:189–195Google Scholar
- 9.Klahr S, Yates J, Bourgoignie J (1971) Inhibition of glycolysis by ethacrynic acid and furosemide. Am J Physiol 221:1038–1043Google Scholar
- 10.Kusakari J, Ise I, Comegys TH, Thalmann I, Thalmann R (1978) Effect of ethacrynic acid, furosemide, and ouabain upon the endolymphatic potential and upon high energy phosphate of the stria vascularis. Laryngoscope 88:12–37Google Scholar
- 11.Mannel MA, Weiner MW (1976) Effects of ethacrynic acid and furosemide on isolated rat kidney mitochondria: inhibition of electron transport in the region of phosphorylation site II. J Pharmacol Exp Ther 198:209–221Google Scholar
- 12.Palheimo S, Thalmann R (1977) Influence of “loop” diuretics upon Na+K+-ATPase and adenylate cyclase of the stria vascularis. Arch Otorhinolaryngol 217:347–359Google Scholar
- 13.Pike DA, Bosher SK (1980) The time course of the strial changes produced by intravenous furosemide. Hearing Res 3:70–89Google Scholar
- 14.Thalmann R, Ise I, Bohne BA, Thalmann I (1977) Action of “loop” diuretics and mercurials upon the cochlea. Acta Otolaryngol 83:221–232Google Scholar
- 15.Thalmann R, Marcus NY, Thalmann I (1978) Adenylate energy charge, energy status, and phosphorylation state of stria vascularis under metabolic stress. Laryngoscope 88:1982–1998Google Scholar
- 16.Yoshida T, Metcoff J (1970) Inhibition of furosemide of glyceroaldehyde-3-phosphate dehydrogenase step in rat kidney. Proc Am Soc Nephrol 4:88Google Scholar
Copyright information
© Springer-Verlag 1985