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Archives of Microbiology

, Volume 147, Issue 3, pp 285–290 | Cite as

Energetic aspects of CO2 uptake in Thiobacillus neapolitanus

  • Y. A. Holthuijzen
  • F. F. M. van Dissel-Emiliani
  • J. G. Kuenen
  • W. N. Konings
Original Papers

Abstract

Uptake of inorganic carbon (Ci) in the form of CO2 and/or HCO 3 - was studied in the chemolithoautotroph Thiobacillus neapolitanus under energy (thiosulphate) or carbon (CO2) limitation. Uptake of C1 was found to be a metabolic energy dependent process since in the presence of uncouplers no uptake was observed. The accumulation level of Ci was higher in the CO2-limited cells (1000-to 1500-fold) in comparison to the thiosulphate-limited cells (500-to 800-fold). The process of uptake could be influenced by addition of ionophores. Inhibition of uptake and accumulation of Ci was found after addition of valinomycin which completely dissipated the electrical potential (ΔΨ). After addition of nigericin an increase in the uptake and accumulation of Ci was observed with a concomitant increase of the ΔΨ. These results suggest that the ΔΨ is the main driving force for uptake of Ci. However, uptake of Ci could never be found in the absence of electron transfer, or in cells in which the electron transfer chain was blocked by potassium cyanide. Electron transfer therefore appears to be an additional requirement for Ci uptake. Kinetic experiment on the uptake of inorganic carbon at different pH values suggest that CO2 is the carbon species taken up by T. neapolitanus.

Key words

Electrochemical proton gradient ΔΨ Electron transfer CO2 uptake thiobacillus neapolitanus 

Abbreviations

RuBisCO

ribulose-1,5-bisphosphate carboxylase

DCCD

N,N1-dicyclohexylcarbodiimide

CCCP

carbonyl cyanide m-chlorophenyl hydrazone

FCCP

carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone

EDTA

sodium ethylene diamine tetraacetate

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Y. A. Holthuijzen
    • 1
  • F. F. M. van Dissel-Emiliani
    • 1
  • J. G. Kuenen
    • 2
  • W. N. Konings
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands
  2. 2.Department of MicrobiologyTechnical UniversityDelftThe Netherlands

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