, Volume 115, Issue 3, pp 207–213 | Cite as

Resistance of Histoplasma capsulatum to killing by human neutrophils

Evasion of oxidative burst and lysosomal-fusion products
  • Nobuyuki Kurita
  • Kiyoshi Terao
  • Elmer Brummer
  • Emiko Ito
  • Kazuko Nishimura
  • Makoto Miyaji


The basis for resistance of yeast form of Histoplasma capsulatum to antifungal activity of human neutrophils was studied. In limiting dilution assays and short term coculture assays human neutrophils were ineffective in killing H. capsulatum whereas Candida albicans was readily killed. By contrast, in a cell free hydrogen peroxide-peroxidase-halide system H. capsulatum was as sensitive to killing as C. albicans. Moreover, lysate of human neutrophils effectively substituted for horse-radish peroxidase in a cell free system for killing H. capsulatum. H. capsulatum elicited significant products of the oxidative burst in human neutrophils as detected by luminol-enhanced chemiluminescence. However, the response was two-fold less (p<0.05) than that induced by C. albicans. Transmission electron microscopy studies showed that phagosome-lysosome fusion took place when neutrophils phagocytosed C. albicans or H. capsulatum. Taken together, these findings indicate that, even though H. capsulatum elicits an oxidative burst and phagosome-lysosome fusion within the phagosome, it is capable of evading damage in short term assays.

Key words

Human PMN H. capsulatum fungicidal resistance 



colony forming units


polymorphonuclear neutrophil


complete tissue culture medium




horseradish peroxidase


lysosomal peroxidase positive material


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Nobuyuki Kurita
    • 1
  • Kiyoshi Terao
    • 1
  • Elmer Brummer
    • 1
  • Emiko Ito
    • 1
  • Kazuko Nishimura
    • 1
  • Makoto Miyaji
    • 1
  1. 1.Research Center for Pathogenic Fungi and Microbial ToxicosesChiba UniversityChibaJapan

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