Archives of Microbiology

, Volume 130, Issue 3, pp 255–261

Selective isolation of Acetobacterium woodii on methoxylated aromatic acids and determination of growth yields

  • Regina Bache
  • Norbert Pfennig
Article

Abstract

Anaerobic enrichments with methoxylated aromatic compounds as substrates (vanillate, syringate, trimethoxycinnamate) were inoculated from freshwater mud and sewage sludge samples. In 12 out of 16 cultures the same type of rod-shaped, motile bacteria was selectively enriched. Two strains, NZva16 and NZva24, were isolated in pure culture and recognized as Acetobacterium woodii by comparison with the type strain (DSM 1030).

All three Acetobacterium strains were able to grow with all 10 of the tested aromatic compounds containing methoxyl groups. In the presence of bicarbonate, these substrates were used as sole organic electron donors and carbon sources. UV-absorption spectra revealed that the aromatic rings were not degraded, and that the corresponding hydroxy derivatives of the methoxylated compounds were formed. The only further fermentation product formed was acetate. When equimolar concentrations of the methoxylated benzoic acid derivatives were applied, the growth yields were proportional to the number of methoxyl groups per molecule. Methoxyl groups or methanol were metabolized by homoacetate fermentation: in the presence of bicarbonate 4 mol of acetate. In case of the methoxylated cinnamic acid derivatives less acetate was formed and the corresponding hydroxy derivatives of phenylpropionic acid appeared as a result of the double bond reduction in the acrylate side chain. In comparison to the benzoate derivatives with the same number of methoxyl groups, higher growth yields were obtained with the cinnamate derivatives.

Key words

Acetobacterium woodii Selective enrichment with methoxylated aromatic acids Anaerobic demethoxylation Methanol Homoacetate fermentation Growth yields Coffeic acid reduction 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Axelrod J, Daly J (1968) Phenol-O-methyltransferase. Biochim Biophys Acta 159:472–478Google Scholar
  2. Balch WE, Schobert S, Tanner RS, Wolfe RS (1977) Acetobacterium, a new genus of hydrogen-oxidizing, carbon dioxide-reducing, anaerobic bacteria. Int J Syst Bacteriol 27:355–361Google Scholar
  3. Bernhardt F-H, Staudinger H, Ullrich V (1970) Eigenschaften einer p-Anisat-O-Demethylase im zellfreien Extrakt von Pseudomonas species. Hoppe-Seyler's Z Physiol. Chem. 351:467–478Google Scholar
  4. Biebl H, Pfennig N (1978) Growth yields of green sulfur bacteria in mixed cultures with sulfur and sulfate reducing bacteria. Arch Microbiol 117:9–16Google Scholar
  5. Braun K (1979) Untersuchungen zum autotrophen, heterotrophen und mixotrophen Wachstrum von Acetobacterium woodii und Clostridium aceticum. Thesis, Univ. GöttingenGoogle Scholar
  6. Braun M (1981) Charakterisierung von anaeroben autotrophen Essigsäurebildnern und Untersuchungen zur Essigsäurebildung aus Wasserstoff und Kohlendioxid durch Clostridium aceticum. Thesis, Univ. GöttingenGoogle Scholar
  7. Cartwright NJ, Buswell JA (1967) The separation of vanillinate O-demethylase from protocatechuate 3,4-oxygenase by ultracentrifugation. Biochem J 105:767–770Google Scholar
  8. Cartwright NJ, Smith ARW (1967) Bacterial attack on phenolic ethers. Biochem J 102:826–841Google Scholar
  9. Hamlett NV, Blaylock BA (1969) Synthesis of acetate from methanol. Bacteriol Proc, p 207Google Scholar
  10. Healy JB Jr, Young LY (1979) Anaerobic biodegradation of eleven aromatic compounds to methane. Appl Environ Microbiol 38:84–89Google Scholar
  11. Imhoff D (1981) Clostridium barkeri: Selen-abhängiger Nikotinsäure-und Purinabbau sowie taxonomische Neueinordnung. Thesis, Univ GöttingenGoogle Scholar
  12. Laanbroek HJ, Pfennig N (1981) Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and in marine sediments. Arch Microbiol 128:330–335Google Scholar
  13. Pfennig N (1978) Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceace. Int J Syst Bacteriol 28:283–288Google Scholar
  14. Smith MR, Mah RA (1978) Growth and methanogenesis by Methanosarcina Strain 227 on acetate and methanoll. Appl Environ Microbiol 36:870–879Google Scholar
  15. Trüper HG, Schlegel HG (1964) Sulphur metabolism in Thiorhodaceae. I. Quantitative measurements of growing cells of Chromatium okenii. Antonie van Leeuwenhoek J Microbiol Serol 30:225–238Google Scholar
  16. Widdel F (1980) Anaerober Abbau von Fettsäuren und Benzoesäure durch neu isolierte Arten Sulfat-reduzierender Bakterien. Thesis, Univ GöttingenGoogle Scholar
  17. Zeikus JG, Lynd LH, Thompson TE, Krzycki JA, Weimer PJ, Hegge PW (1980) Isolation and characterization of a new, methylotrophic, acidogenic anaerobe, the Marburg strain. Curr Microbiol 3:381–386Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Regina Bache
    • 1
  • Norbert Pfennig
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzFederal Republic of Germany

Personalised recommendations