Archives of Microbiology

, Volume 152, Issue 2, pp 115–118

Pyrimidine, purine and nitrogen control of cytosine deaminase synthesis in Escherichia coli K12. Involvement of the glnLG and purR genes in the regulation of codA expression

  • Lennart Andersen
  • Mogens Kilstrup
  • Jan Neuhard
Original Papers

Abstract

Cytosine deaminase, encoded by the codA gene in Escherichia coli catalyzes the deamination of cytosine to uracil and ammonia. Regulation of codA expression was studied by determining the level of cytosine deaminase in E. coli K12 grown in various defined media. Addition of either pyrimidine or purine nucleobases to the growth medium caused repressed enzyme levels, whereas growth on a poor nitrogen source such as proline resulted in derepression of cytosine deaminase synthesis. Derepression of codA expression was induced by starvation for either uracil or cytosine nucleotides. Nitrogen control was found to be mediated by the glnLG gene products, and purine repression required a functional purR gene product. Studies with strains harbouring multiple mutations affecting both pyrimidine, purine and nitrogen control revealed that the overall regulation of cytosine deaminase synthesis by the different metabolites is cumulative.

Key words

Escherichia coli codA glnLG purR Gene regulation Cytosine deaminase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beck CF, Ingraham JL, Neuhard J (1972a) Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. Mol Gen Genet 115:208–215Google Scholar
  2. Beck CF, Ingraham JL, Neuhard J, Thomassen E (1972b) Metabolism of pyrimidines and pyrimidine nucleotides by Salmonella typhimurium. J Bacteriol 110:219–228Google Scholar
  3. Chen Y-M, Backman K, Magasanik B (1982) Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli. J Bacteriol 150:214–220Google Scholar
  4. Clark DJ, Maaløe O (1967) DNA replication and the division cycle in Escherichia coli J Mol Biol 23:99–112Google Scholar
  5. Gutnick D, Calvo JM, Klopotowski I, Ames BN (1969) Compounds which serve as the sole source of carbon or nitrogen for Salmonella typhimurium LT-2. J Bacteriol 100:215–219Google Scholar
  6. Haan PG de, Felix HS, Peters R (1972) Mapping for the gene for cytosine deaminase on the Escherichia coli chromosome. Antonie van Leeuwenhoek J Microbiol Serol 38:257–263Google Scholar
  7. Kilstrup M, Meng LM, Neuhard J, Nygaard P (1989) Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthesis enzymes in Escherichia coli. J Bacteriol 171:2124–2127Google Scholar
  8. Magasanik B, Neidhardt FC (1987) Regulation of carbon and nitrogen utilization. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger, HE (eds) Escherichia coli and Salmonella typhimurium. Cellular and molecular biology. Am Soc Microbiol, Washington, DCGoogle Scholar
  9. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  10. Neuhard J (1968) Pyrimidine nucleotide metabolism and pathways of thymidine triphosphate biosynthesis in Salmonella typhimurium. J Bacteriol 96:1519–1527Google Scholar
  11. Neuhard J, Ingraham J (1968) Mutants of Salmonella typhimurium requiring cytidine for growth. J Bacteriol 95:2431–2433Google Scholar
  12. Rasmussen UB, Mygind B, Nygaard P (1986) Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12. Biochim Biophys Acta 881:268–275Google Scholar
  13. Schwartz M, Neuhard J (1975) Control of expression of the pyr genes in Salmonella typhimurium: Effects of variations in uridine and cytidine nucleotide pools. J Bacteriol 121:814–822Google Scholar
  14. West TP, O'Donovan GA (1982) Repression of cytosine deaminase in Salmonella typhimurium. J Bacteriol 149:1171–1174Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Lennart Andersen
    • 1
  • Mogens Kilstrup
    • 1
  • Jan Neuhard
    • 1
  1. 1.Institute of Biological Chemistry BUniversity of CopenhagenCopenhagen KDenmark
  2. 2.NOVO Industries A/SBagsværdDenmark

Personalised recommendations