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The experimental study of scoliosis in bipedal rat in lathyrism

  • Haruhito Tanaka
  • Yoji Kimura
  • Yoichi Ujino
Original Works

Summary

Using Wister albino rats, bipedal rats were prepared; to which semicarbazide at a concentration of 0.075%, 0.05% and 0.025% were given as drinking water and the rats were divided into three groups A, B and C according to such concentrations.

Osteolathyrism was caused in this way and the relations between doses as well as the dosing period and the progress of scoliosis were examined roentgenologically and histologically and the following conclusions were obtained.
  1. 1.

    The frequency of occurrence of scoliosis was 82% in the bipedal rats and 13% in the quadrupedal rats (level of significance α=0.001% or less).

     
  2. 2.

    Primordial top vertebrae were located concentratively in the lower 1/3 of thoracic vertebra.

     
  3. 3.

    In the primordial curve, those cases showing a right side curve accounted for 76%.

     
  4. 4.

    After termination of administration of semicarbazide, spontanneous improvement took place only in those cases showing scoliosis of less than 40°.

     
  5. 5.

    In the author's experimental scoliosis, the static and dynamic condition of posture nearly coincided with those of men in clinical studies.

     
  6. 6.

    Histological changes were especially marked in the cartilage layer on the vertebral epiphyseal line, being quite diversified.

     

Keywords

Drinking Water Thoracic Vertebra Semicarbazide Dose Period Cartilage Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Wister-Albino-Ratten, die bipedal präpariert wurden, ist Trinkwasser verabreicht worden, welches Semikarbazid in einer Konzentration von jeweils 0,075, 0,05 und 0,025% enthielt. Entsprechend diesen Konzentrationen wurden die Versuchstiere in die Gruppen A, B und C aufgeteilt.

Auf these Weise wurde ein Osteolathyrismus hergestellt, und in Abhängigkeit von der Dosis und der Verabreichungsdauer wurde das Fortschreiten der Skoliose röntgenologisch und histologisch untersucht. Folgende Erkenntnisse wurden gewonnen:
  1. 1.

    Die Häufigkeit der Skoliose betrug bei den bipedalen Ratten 82% und bei den quadrupedalen Batten 13% (das Signifikanzniveau α=0,001% oder niedriger).

     
  2. 2.

    Der Krümmungsscheitel lag im unteren Drittel der Brustwirbelsdule.

     
  3. 3.

    Die Primärkrümmung war in 76% rechtskonvex.

     
  4. 4.

    Nach Beendigung der Semikarbazid-Verabreichung trat eine Spontanbesserung nur in den Fällen auf, die einen skoliotischen Krümmungswinkel von weniger als 40° aufwiesen.

     
  5. 5.

    Bei den experimentellen Skoliosen stimmten die statischdynamischen Haltungsverhältnisse nahezu vollständig mit denen beim Menschen überein.

     
  6. 6.

    Die histologischen Veränderungen waren besonders auffallend und mannigfaltig in den Epiphysenknorpeln der Wirbel.

     

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References

  1. Arnd C (1903) Experimentelle Beiträge der Skoliose. Arch Ortop 1:145Google Scholar
  2. Alvin M, Simon M (1950) Radiation scoliosis an experimental study. J Bone Jt Surg 32-A:396Google Scholar
  3. Arkin AM (1950) The mechanism of rotation in combination with lateral deviation in the normal spine. J Bone Jt Surg 32-A:180Google Scholar
  4. Acton HW (1922) An investigation into the causation of lathyrism in man. Indian Medical Gazette 7:241Google Scholar
  5. Amato VP (1969) Early skeletal lesion and vascular changes in rats. J Bone Jt Surg 41-A:600Google Scholar
  6. Bradford EH (1893) Treatment of lateral curvature by means of pressure correction. Bost Med Surg J 128:463Google Scholar
  7. Bisgard JD (1940) Scoliosis its experimental production and growth correction. Growth and fusion of vertebral bodies. Surg Gynec Obstet 70:1029Google Scholar
  8. Dupey HW (1954) Isolation of material capable of producing experimental lathyrism. J Am Pharm Ass (Scientific ed.) 43:61Google Scholar
  9. Dasler W (1958) Production by semicarbazide of gross skeletal changes in rats similar to osteolathyrism. Proc Exp Biol Med 97:112Google Scholar
  10. Epstein BS (1962) The spine. A radiological text and Atlas. Lea & Febiger, PhiladelphiaGoogle Scholar
  11. Ferguson A (1949) Roentgen diagnosis of extremities and spine, 2nd ed. Paul B. Hoeber, New YorkGoogle Scholar
  12. Goldwait J (1916) Opportunity for the orthopaedic in preventative medicine through educational work on posture. Am J Orthop Surg 14:443Google Scholar
  13. Hass S (1939) Experimental production of scoliosis. J Bone Jt Surg 21:963Google Scholar
  14. Jimenz-Diaz C (1942) Inveestigation sobre et latirismo. II. Rev Clin Espan 5:168Google Scholar
  15. Kostwik J (1973) Halop-pelvic traction in the surgical management of adult scoliosis. J Bone Jt Surg 52-B:232Google Scholar
  16. Knutsson F (1963) A contribution to discussion of the biological cause of idiopathic scoliosis. Acta Orthop Scand 33:98Google Scholar
  17. Langer F (1913) Die Skoliose. Erg Chir Orthop 7:748Google Scholar
  18. Müller W (1928) Skoliosen im Tierversuch. Burn's Beitr Klin Chir 142:343Google Scholar
  19. Mehta MH (1973) Radiation estimation of vertebral rotation in scoliosis. J Bone Jt Surg 55-A:513Google Scholar
  20. Nachlas (1Q950) Experimental scoliosis. Surg Gynec Obstet 90: 672Google Scholar
  21. Nach C (1969) A study vertebral rotation. J Bone Jt Surg 51A:223Google Scholar
  22. Ponseti T (1954) Lesion of skeleton and other mesodermal tissue in rats fed sweet pea (Lathyrus odoratus) seeds. J Bone Jt Surg 36-A:1631Google Scholar
  23. Perry O (1962) Idiopathic scoliosis. Acta Orthop Scand 32:39Google Scholar
  24. Pitzen P (1928) Experimentelle Erzeugung von Skoliose. Z Orthop Chir 49:58Google Scholar
  25. Pacher W (1939) Operative Erzeugung einer Skoliose im Tierversuch. Z Orthop 69:140Google Scholar
  26. Rodriguez R (1965) Skeletal lesion of lathyrism and effects bipedalism on spine development. Clin Orthop 41:189Google Scholar
  27. Risser J (1958) A follow-up study of treatement of scoliosis. J Bone Jt Surg 40-A:555Google Scholar
  28. Ramamurti P (1959) Skeletal lesion produced by semicarbazide and experimental analysis of the action lathyrogenic compounds. J Bone Jt Surg 41-A:590Google Scholar
  29. Stanley H (1973) Histochemical finding in the paraspinal muscles of patients with idiopathic scoliosis. J Bone Jt Surg 55-A:435Google Scholar
  30. Sakamoto K (1958) Study on bone deformation using bipedal rats. Shikoku Med J 14:1149Google Scholar
  31. Somerville E (1952) Rotational lordosis. The development of single curve. J Bone Jt Surg 34-A:421Google Scholar
  32. Tsuji H (1963) Experimenteal study on scoliosis. Chiba Med J 39:45Google Scholar
  33. Tadoloro (1962) Body-weight and weight of organs of rats. North Kanto Med J 12:250Google Scholar
  34. Yamada K (1965) Static and dynamic studies scoliosis. Progress of Orthopaedic Surgery. Nankodo, TokioGoogle Scholar
  35. Wellstein I (1902) Die Skoliose in ihrer Behandlung und Entstehung nach klinischen und experimentellen Studien. Z Orthop 10:117Google Scholar
  36. Zambotti V (1957) The biochemistry of presseous cartilage and ossification. Scienta Med Ital (English Ed.) 5:614Google Scholar

Copyright information

© J. F. Bergmann Verlag 1982

Authors and Affiliations

  • Haruhito Tanaka
    • 1
  • Yoji Kimura
    • 1
  • Yoichi Ujino
    • 1
  1. 1.Department of InstituteLaboratory Orthopaedic SurgeryMatsuyama city, Ootemachil-1-1, Matsumadaiichi buildJapan

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