Organ of Corti-specific polypeptides: OCP-I and OCP-II
- 34 Downloads
Two polypeptides, OCP-I and OCP-II, specific to the organ of Corti of the guinea pig and the rat, were detected by two-dimensional polyacrylamide gel electrophoresis. They were neither present in other inner ear structures, nor in other organs tested. The two polypeptides constitute a substantial portion of the total protein of the organ of Corti. Both inner and outer layer of the organ of Corti contained approximately equal amounts of OCP-I and OCP-II. The molecular weights of OCP-I and OCP-II are 37,000 and 22,500 daltons, respectively. The corresponding pI values are 4.9 and 5.1, respectively. In addition, actin and tubulin were detected in both layers of the organ of Corti.
Key wordsOrgan of Corti Organ-specific polypeptides Two-dimensional polyacrylamide gel electrophoresis Guinea pig Rat Actin
Unable to display preview. Download preview PDF.
- 1.Moore BW (1976) Brain-specific proteins: S-100 protein, 14-3-2 protein, and glial fibrillary protein. In: Agranoff BW, Aprison MH (eds) Adv Neurochem, vol 1. Plenum, New York, pp 137–155Google Scholar
- 2.Moore BW (1980) Chemistry and biology of the S-100 protein. Scand J Immunol (in press)Google Scholar
- 3.Thalmann R (1976) Quantitative histo- and cytochemistry of the ear. In: Smith CA, Vernon JA (eds) The handbook of auditory and vestibular research methods. Part III Biochemistry. Thomas, Springfield, IL, pp 359–419Google Scholar
- 4.O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021Google Scholar
- 5.Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685Google Scholar
- 6.Angelborg C, Engstrom H (1973) The normal organ of Corti. In: Møller AR (ed) Basic mechanisms in hearing. Academic Press, New York London, pp 125–183Google Scholar
- 7.Dallos P (pers commun)Google Scholar
- 8.Flock A, Cheung H, Wersäll J (1979) Pathological actin in vestibular hair cells of the waltzing guinea pig. In: Stahle J, Pfaltz CR (eds) Adv. Oto-Rhino-Laryng, vol 25. Karger, Basel, pp 12–16Google Scholar