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Archives of Microbiology

, Volume 144, Issue 1, pp 78–83 | Cite as

ATP-driven succinate oxidation in the catabolism of Desulfuromonas acetoxidans

  • Jens Paulsen
  • Achim Kröger
  • Rudolf K. Thauer
Original Papers

Abstract

The oxidation of succinate with elemental sulphur in Desulfuromonas acetoxidans was investigated using a membrane preparation of this bacterium. The following results were obtained:
  1. 1.

    The preparation catalyzed the oxidation of succinate with sulphur and NAD. These reactions were dependent on ATP and were abolished by the presence of protonophores or dicyclohexylcarbodiimide (DCCD).

     
  2. 2.

    The membrane preparation also catalyzed the reduction of fumarate with H2S or with NADH. These activities were not dependent on ATP and were not affected by protonophores or DCCD.

     
  3. 3.

    By extraction-reincorporation experiments it could be shown that menaquinone is involved in electron transport between H2S and fumarate and between NADH and fumarate.

     
  4. 4.

    The membrane fraction catalyzed the reduction of the water-soluble menaquinone-analogue dimethylnaphthoquinone (DMN) by succinate, H2S, or NADH, and the oxidation of DMNH2 by fumarate. These activities were not dependent on the presence of menaquinone and were not influenced by ATP.

     
  5. 5.

    The activities involving succinate oxidation or fumarate reduction were similarly sensitive to 2(n-nonyl)-4-hydroxyquinoline-N-oxide, while H2S and NADH oxidation by DMN were not affected by the inhibitor.

     

It is concluded that the catabolism of D. acetoxidans involves the energy-driven oxidation of succinate with elemental sulphur or NAD as electron acceptors and that menaquinone is a component of the electron transport chain catalyzing these reactions.

Key words

Desulfuromonas acetoxidans Succinate oxidation Sulphur reduction Acetate oxidation Citric acid cycle Reverse of electron transport Menaquinone ATP synthase Succinate dehydrogenase Sulphur reductase NADH dehydrogenase 

Non-standard abbreviations

DMN

2,3-Dimethyl-1,4-naphthoquinone

TTFB

4,5,6,7-Tetrachloro-2′-trifluoromethylbenzimidazole

NQNO

2(n-Nonyl)-4-hydroxyquinoline-N-oxide

DCCD

N,N′-Dicyclohexylcarbodiimide

APAD

3-Acetylpyridineadenine-dinuclcotide

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Copyright information

© Springer-Verag 1986

Authors and Affiliations

  • Jens Paulsen
    • 1
  • Achim Kröger
    • 1
  • Rudolf K. Thauer
    • 1
  1. 1.Fachbereich Biologie der UniversitätMarburgGermany

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