Archives of Microbiology

, Volume 140, Issue 2–3, pp 101–106 | Cite as

Botryococcus braunii carbon/nitrogen metabolism as affected by ammonia addition

  • Masayuki Ohmori
  • Fred R. Wolf
  • James A. Bassham
Original Papers

Abstract

Carbon metabolism in photosynthesizing and respiring cells of Botryococcus braunii was radically changed by the presence of 1 mM NH4Cl in the medium, when the so-called “resting state” previously had been subjected to a nitrogen-deficient medium. Ammonia addition to the algae photosynthesizing with 14C-labelled HCO 3 - almost completely inhibited the synthesis of 14C-labelled botryococcenes and other hexane-extractable compounds, and also inhibited the formation of insoluble compounds; however, it resulted in a large increase in the synthesis of alanine, glutamine, other amino acids, and especially of 5-aminolevulinic acid. Total CO2 fixation decreased about 60% and O2 evolution decreased more than 50%.

CO2 fixation in the dark with ammonia present led to labelled products derived from phosphoenolpyruvate carboxylation, such as glutamine, glutamate, and malate. Respiratory uptake of O2 increased by about 70%.

The inhibition of terpenoid synthesis and increased synthesis of C5 amino acids by Botryococcus upon ammonia addition indicates 1) a diversion of acetyl coenzyme A from synthetic pathways leading to terpenoids and 2) increased operation of pathways leading to the synthesis of amino acids, especially 5-aminolevulinic acid, a precursor to chlorophyll biosynthesis.

Key words

5-aminolevulinic acid Ammonia botryococcus braunii Botryococcenes Carbon Hydrocarbon Metabolism Nitrogen Resting state Terpenoids 

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References

  1. Beale SI, Gouth SP, Granick S (1975) Biosynthesis of δ-aminolevulinic acid from the intact carbon skeleton of glutamic acid in greening barley. Proc Natl Acad Sci USA 72:2719–2723Google Scholar
  2. Brown AC, Knights BA, Conway E (1969) Hydrocarbon content and its relationship to physiological state in the green alga Botryococcus braunii. Phytochemistry 8:543–547Google Scholar
  3. Bruinsma J (1963) The quantitative analysis of chlorophylls a and b in plant extracts. Photochem Photobiol (Chlor Metabol Sym) 2:241–249Google Scholar
  4. Cox RE, Burlingame AL, Wilson DM, Eglington G, Maxwell JR (1973) Botryococcene- a tetramethylated acyclic triterpenoid of algal origin. J C S Chem Comm 284–285Google Scholar
  5. Gelpi E, Oro J, Schneider HJ, Bennett EO (1968) Olefins of high molecular weight in two microscopic algae. Science 161:700–702Google Scholar
  6. Hammel KE, Cornwell KL, Bassham JA (1979) Stimulation of dark CO2 fixation by ammonia in isolated mesophyll cells of Papaver somniferum L. Plant Cell Physiol 20:1523–1529Google Scholar
  7. Harel H, Ne'eman E (1983) Alternate routes for the synthesis of 5-aminolevulinic acid in maize leaves. Plant Physiol 72:1062–1067Google Scholar
  8. Kanazawa T, Kirk MR, Bassham JA (1970) Regulatory effects of ammonia on carbon metabolism in photosynthesizing Chlorella pyrenoidosa. Biochim Biophys Acta 205:401–408Google Scholar
  9. Kanazawa T, Kanazawa K, Kirk MR, Bassham JA (1972) Regulatory effects of ammonia on carbon metabolism in Chlorella pyrenoidosa during photosynthesis and respiration. Biochim Biophys Acta 226:656–669Google Scholar
  10. Kanazawa T, Distefano M, Bassham JA (1983) Ammonia regulation of intermediary metabolism in photosynthesizing and respiring Chlorella pyrenoidosa: Comparative effects of methylamine. Plant & Cell Physiol 24(6):979–986Google Scholar
  11. Keys AJ, Bird IF, Cornelius MJ, Lea PJ, Wallsgrove RM, Miflin BJ (1978) Photorespiratory nitrogen cycle. Nature 275:741–743Google Scholar
  12. Knights BA, Brown AC, Conway E (1970) Hydrocarbons from the green form of the freshwater alga Botryococcus braunii. Phytochemistry 9:1317–1324Google Scholar
  13. Largeau C, Casadevall E, Dif D, Berkaloff C (1980) Renewable hydrocarbon production from the alga Botryococcus braunii. In: Üalz W, Chartier P, Hall DO (eds) Energy from biomass, First European Conference. Applied Science Publishers Ltd., London, England pp 653–658Google Scholar
  14. Mauzerall D, Granick S (1956) The occurrence and determination of δ-aminolevulinic acid and porphobilinigen in urine. J Biol Chem 219:435–446Google Scholar
  15. Meller E, Belkin S, Harel E (1975) The biosynthesis of δ-aminolevulinic acid in greening maize leaves. Phytochemistry 14:2399–2402Google Scholar
  16. Niklas KJ (1976) Chemical examination of some non-vascular paleozoic plants. Brittonia 28:113–137Google Scholar
  17. Pedersen TA, Kirk M, Bassham JA (1966) Light-dark transients in levels of intermediate compounds during photosynthesis in air-adapted Chlorella. Physiol Plantarum 19:219–231Google Scholar
  18. Wake LV, Hillen LW (1981) Nature and hydrocarbon content of blooms of the alga Botryococcus braunii occurring in Australian freshwater lakes. Aust J Mar Freshwater Res 32:353–367Google Scholar
  19. Wolf FR (1981) The ultrastructure and hydrocarbons of Botryococcus braunii Kutzing (Chlorophyceae). PhD Thesis, Texas A&M UniversityGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Masayuki Ohmori
    • 1
  • Fred R. Wolf
    • 1
  • James A. Bassham
    • 1
  1. 1.Laboratory of Chemical Biodynamics, Lawrence Berkeley LaboratoryUniversity of CaliforniaBerkeleyUSA

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