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Archives of Microbiology

, Volume 151, Issue 6, pp 486–490 | Cite as

Identification of aggregation substances of Enterococcus faecalis cells after induction by sex pheromones

An immunological and ultrastructural investigation
  • Dominique Galli
  • Reinhard Wirth
  • Gerhard Wanner
Original Papers

Abstract

The sex pheromone system of Enterococcus faecalis is responsible for the clumping response of a plasmid carrying donor strain with a corresponding plasmid free recipient strain due to the production of sex pheromones by the recipient strain. The clumping response is mediated by a surface material (called aggregation substance) which is synthesized upon addition of sex pheromones to the cultures. Here we show that after induction a dense layer of “hairlike” structures is formed on the cell wall of the bacteria. These hairlike structures are responsible for the cell-cell contact which leads to the aggregation of cells. Formation of these structures was specific, only occurring after the addition of homologous sex pheromone.

Key words

Aggregation substance Enterococcus faecalis Sex pheromone system Transmission electron microscopy Immunogold labelling technique 

Abbreviations

cAD1

sex pheromone specific for plasmid pAD1

cPD1

sex pheromone specific for plasmid pPD1

CW

cell wall

pAD1

conjugative plasmid specifically transferred in the presence of cAD1

pPD1

conjugative plasmid specifically transferred in the presence of cPD1

PBS

10 mM Na-phosphate pH 7.5, 0.85% NaCl

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Dominique Galli
    • 1
  • Reinhard Wirth
    • 1
  • Gerhard Wanner
    • 2
  1. 1.Lehrstuhl für Mikrobiologie der Universität MünchenMünchen 19Germany
  2. 2.Botanisches Institut der Universität MünchenMünchen 19Germany

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