Planta

, Volume 145, Issue 3, pp 293–303

Assay for and enzymatic formation of an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid

  • Thomas Boller
  • Robert C. Herner
  • Hans Kende
Article

DOI: 10.1007/BF00454455

Cite this article as:
Boller, T., Herner, R.C. & Kende, H. Planta (1979) 145: 293. doi:10.1007/BF00454455

Abstract

A simple and sensitive chemical assay was developed for 1-aminocyclopropane-1-carboxylic acid (ACC), a precursor of ethylene. The assay is based on the liberation of ethylene from ACC at pH 11.5 in the presence of pyridoxal phosphate, MnCl2 and H2O2. This assay was used to detect ACC in extracts of tomato fruits (Lycopersicon esculentum Mill.) and to measure the activity of a soluble enzyme from tomato fruit that converted S-adenosylmethionine (SAM) to ACC. The enzyme had a Km of 13 μM for SAM, and conversion of SAM to ACC was competitively and reversibly inhibited by aminoethoxyvinylglycine (AVG), an analog of rhizobitoxine. The Ki value for AVG was 0.2 μM. The level of the ACC-forming enzyme activity was positively correlated with the content of ACC and the rate of ethylene formation in wild-type tomatoes of different developmental stages. Mature fruits of the rin (non-ripening) mutant of tomato, which only produce low levels of ethylene, contained much lower levels of ACC and of the ACC-forming enzyme activity than wild-type tomato fruits of comparable age.

Key words

1-Aminocyclopropane-1-carboxylic acid Ethylene Lycopersicon Mutant, rin (tomato) Rhizobitoxine analog 

Abbreviations

ACC

1-aminocyclopropane-1-carboxylic acid

AVG

ammoethoxyvinylglycine, the aminoethoxy analog of rhizobitoxine L-2-amino-4-(2′-aminoethoxy)-trans-3-butenoic acid

SAM

S-adenosyl-L-methionine

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Thomas Boller
    • 1
  • Robert C. Herner
    • 2
  • Hans Kende
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  2. 2.Department of HorticultureMichigan State UniversityEast LansingUSA
  3. 3.Botanisches Institut der Universität BaselBaselSwitzerland

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