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Changes in protein synthesis during the initial stage of life of tobacco protoplasts

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The biosynthesis of Fraction I protein in isolated protoplasts is compared with that in the plant. Radioactive precursors were incorporated into isolated protoplasts (“in vitro” labeling) and into leaves, from which the protoplasts were isolated later (“in situ” labeling). The biosynthesis of Fraction I protein stopped almost completely as soon as the protoplasts were incubated in the culture medium.

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Fleck, J., Durr, A., Lett, M.C. et al. Changes in protein synthesis during the initial stage of life of tobacco protoplasts. Planta 145, 279–285 (1979). https://doi.org/10.1007/BF00454453

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Key words

  • Fraction I protein
  • Nicotiana
  • Protein synthesis
  • Protoplasts