Keratin debris is a constant feature in middle-ear cholesteatoma. Keratin prepared from rat skin induced a foreign-body granuloma in the subcutaneous space in the rat. In vitro this granuloma produced high levels of bone-resorbing factors: prostaglandin E2, osteoclast-activating factor, and leucine aminopeptidase. In the in vivo study, keratin-induced granuloma in the rat middle ear caused partial resorption of the cochlear wall. Macrophages, fibroblasts, and osteoclastlike cells were found at bone-resorption areas. These cells appeared to be responsible for bone resorption through production of prostaglandin E2, osteoclast-activating factor, and proteases.
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Supported by the Deafness Research Foundation
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Moriyama, H., Huang, C.C., Shirahata, Y. et al. Effects of keratin on bone resorption in experimental otitis media. Arch Otorhinolaryngol 239, 61–68 (1984). https://doi.org/10.1007/BF00454263
- Bone resorption
- Keratin-Prostaglandin E2
- Osteoclast-activating factor