Archives of oto-rhino-laryngology

, Volume 244, Issue 1, pp 61–65

Cation transport in the ampulla of the semicircular canal and in the endolymphatic sac

  • N. Mori
  • O. Ninoyu
  • C. Morgenstern


We examined the effects of anoxia and ethacrynic acid on the endolymphatic potential and cation activity in the superior ampulla of the guinea pig, using double-barrelled ion-exchanger microelectrodes. In normal guinea pigs the ampullar endolymphatic potential was +3.9±1.2 mV (n=32), the Cl activity 130±4.6 mM (n=9), and the Na+ activity 18.4±4.4 mM (n=20). After anoxia, the ampullar DC potential decreased rapidly and reversed its polarity within 5 min. It then decreased gradually for 60 min and increased afterwards to approximately zero. K+ activity decreased gradually after a latency of 10 min, whereas Na+ activity increased. During the gradual decrease of a negative ampullar endolymphatic potential, an increase in Na+ activity was observed. Thirty minutes after the intravenous injection of ethacrynic acid (100 mg/kg), the potential began to decrease, changed to a negative polarity, and approached a maximum negative level 100 min after the injection. The decrease in K+ activity corresponded to the reduction of potential whereas Na+ activity remained unchanged. The DC potential of the endolymphatic sac in normal guinea pigs was + 14.7±5.1 mV (n=17). The Na+ concentration was 103.3±14.7 mM (n=14) and the K+ concentration was 11.6 ±0.8 mM (n=4). After anoxia, the DC potential decreased rapidly and approached 0 mV within 8 min. No negative potential could be observed. The Na+ concentration began to increase 2 min after anoxia and reached the extracellular Na+ concentration about 30 min later. No significant effect of intravenous administration of ethacrynic acid (100 mg/kg) on DC potential and Na + concentration could be observed. The results suggest the presence of a different ion transport system in the endolymphatic sac from that of the cochlea and the ampullae of the semicircular canals.

Key words

Na+ activity K+ activity DC potential Semicircular canal Endolymphatic sac 


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  1. 1.
    Amano H, Orsulakova A, Morgenstern C (1983) Intracellular and extracellular ion content of the endolymphatic sac. Arch Otorhino 237:273–277Google Scholar
  2. 2.
    Eldrege DH (1961) The electrical polarization of the semicircular canals (guinea pigs). Ann Otol 70:1024–1036Google Scholar
  3. 3.
    Frizzell RA, Field M, Schaltz SG (1979) Sodium —coupled chloride transport by epithelial tissues. Am J Physiol 236:1–8Google Scholar
  4. 4.
    Guild SR (1927) The circulation of endolymph. Am J Anat 39:57–81Google Scholar
  5. 5.
    Güggi M, Ohme M, Pretsch E, Simon W (1976) Neutraler lonophor für Flüssigmembranelectroden mit hoher Selektivität für Natrium — gegenüber Kaliumionen. Helv Chim Acta 54:2417–2420Google Scholar
  6. 6.
    Kimura RS, Schuknecht HF (1965) Membranous hydrops in the inner ear of the guinea pigs after obliteration of the endolymphatic sac. Pract Otorhinolaryngol 27:343–354Google Scholar
  7. 7.
    Kusakari I, Thalmann R (1976) Effects of anoxia and ethacrynic acid upon ampullar endolymphatic potential and upon high energy phosphate in ampullar wall. Laryngoscope 86:132–147Google Scholar
  8. 8.
    Lundquist PG (1965) The endolymphatic duct and sac in the guinea pis. An electron microscopic and experimental investigation. Acta Otolaryngol (Stockh) [Suppl] 201:100–108Google Scholar
  9. 9.
    Marcus DC, Marcus NY (1985) Transepithelial measurements of electrical potential and potassium in the dark cell region of the utricle. Inner Ear Biology Meeting, Würzburg, p 84Google Scholar
  10. 10.
    Makimoto K, Takeda T, Silverstein H (1980) Species differences in inner ear fluids. Arch Otorhinolaryngol 228:187–193Google Scholar
  11. 11.
    Miyamoto HC, Morgenstern C (1979) Potassium level in endolymphatic sac of the guinea pig in vivo. Arch Otorhinolaryngol 222:77–78Google Scholar
  12. 12.
    Nakumura T (1967) Experimental obliteration of the endolymphatic sac and the perilymphatic duct (histological and biochemical studies). J Otorhinolaryngol Soc Jpn 70: 932–941Google Scholar
  13. 13.
    Ninoyu O, Meyerzum Gottesberge AM (1986) Ca++ activity in the endolymphatic space. Acta Otolaryngol (Stockh) 102:222–227Google Scholar
  14. 14.
    Rask-Andersen H, Bredberg G, Lyttkens L, Lööf G (1981) The function of the endolymphatic duct: an experimental study using ionic lanthanum as a tracer: a preliminary report. Ann NY Acad Sci 374:11–19Google Scholar
  15. 15.
    Schmidt RS (1963) Independence of the endovestibular potential in homeotherma. J Gen Physiol 47:371–378Google Scholar
  16. 16.
    Sellick PM, Johnstone BM (1972) The electrophysiology of the utricle. Pflügers Arch 336:21–27Google Scholar
  17. 17.
    Sellick PM, Johnstone BM (1974) Differential effects of ouabain and ethacrynic acid on the labyrinthine potential. Pflügers Arch 352:339–350Google Scholar
  18. 18.
    Sellick PM, Johnstone BM (1975) Production and role of inner ear fluid. Prog Neurobiol 5:337–362Google Scholar
  19. 19.
    Silverstein H (1966) Biochemical and physiological studies of the endolymphatic sac in the cat. Laryngoscope 76:498–512Google Scholar
  20. 20.
    Silverstein H, Takeda T (1977) Endolymphatic sac obstruction. Biochemical studies. Ann Otol 86:493–499Google Scholar
  21. 21.
    Spring KR, Kimura G (1978) Chloride reabsorption by renal proximal tubules of Necturus. J Membr Biol 38:233–254Google Scholar
  22. 22.
    Zeuthen T (1976) Gradients of chemical and electrical potentials in the gall-bladder. J Physiol 254:32Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • N. Mori
    • 1
  • O. Ninoyu
    • 1
  • C. Morgenstern
    • 1
  1. 1.ENT ClinicUniversity of DüsseldorfDüsseldorf 1Federal Republik of Germany
  2. 2.Department of OtolaryngologyOsaka State UniversityJapan
  3. 3.Department of OtolaryngologyKansai Medical UniversityOsakaJapan

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