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Applied Microbiology and Biotechnology

, Volume 27, Issue 5–6, pp 521–527 | Cite as

Stability during fermentation of a recombinant α-amylase plasmid in Bacillus subtilis

  • Alexis Harington
  • Terence G. Watson
  • Maureen E. Louw
  • Jill E. Rodel
  • Jennifer A. Thomson
Applied Genetics and Regulation

Summary

We have studied the stability during fermentation of a hybrid plasmid carrying a Bacillus α-amylase gene in Bacillus subtilis. In the absence of antibiotic selection plasmid loss was associated largely with the post-exponential phases of growth and decline. In fermentations containing selective antibiotics, various deleted plasmids were recovered during late stationary phase, regardless of whether the host was rec+ or recE. We therefore propose that the plasmid loss observed during late growth in antibiotic-free fermentations is due to deletion events which include the origin of plasmid replication. The structure of the deleted plasmids was determined and the sequences in the vicinity of the end-points analysed. When the deleted plasmids were subjected to further fermentations in the absence of selective antibiotics, they were completely stable.

Keywords

Fermentation Bacillus Stationary Phase Bacillus Subtilis Late Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Alexis Harington
    • 1
  • Terence G. Watson
    • 2
  • Maureen E. Louw
    • 2
  • Jill E. Rodel
    • 3
  • Jennifer A. Thomson
    • 4
  1. 1.CSIR Laboratory for Molecular and Cell BiologyJohannesburg
  2. 2.CSIR National Food Research InstitutePretoriaSouth Africa
  3. 3.Department of Membrane ResearchWeizmann Institute of ScienceRehovotIsrael
  4. 4.CSIR Laboratory for Molecular and Cell BiologyBraamfonteinSouth Africa

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