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Mass production of lipase by fed-batch culture of Pseudomonas fluorescens


High concentration production of an extracellular enzyme, lipase, was achieved by a fed-batch culture of Pseudomonas fluorescens. During the cultivation, temperature, pH and dissolved oxygen concentration wwre maintained at 23°C, 6.5 and 2–5 ppm, respectively. Olive oil was used as a carbon source for microbial growth. To produce lipase effectively the specific feed rate of olive oil had to be maintained in a range of 0.04–0.06 (g oil) · (g dry cell)-1 · h-1. The CO2 evolution rate was monitored to estimate the requirement of olive oil. The ratio of feed rate of olive oil to the CO2 evolution rate was varied in the range of 20–60 g oil/mol CO2. The higher value of the ratio accelerated microbial growth, but did not favour lipase production. Once the high cell concentration of 60 g/l had been achieved, the ratio was changed from 50 to 30 g oil/mol CO2 to accelerate the lipase production. By this CO2-dependent method a very high activity of lipase, 1980 units/ml, was obtained. Both the productivity and yield of lipase were prominently increased compared with a conventional batch culture.

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Correspondence to Tsuneo Yamane.

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Suzuki, T., Mushiga, Y., Yamane, T. et al. Mass production of lipase by fed-batch culture of Pseudomonas fluorescens . Appl Microbiol Biotechnol 27, 417–422 (1988).

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  • Lipase
  • Dissolve Oxygen
  • Feed Rate
  • Batch Culture
  • Microbial Growth