Archives of Dermatological Research

, Volume 262, Issue 3, pp 323–328 | Cite as

Allotransplantation of cultured human skin

  • A. M. P. Janssen de Limpens
  • R. H. Cormane
  • A. H. Siddiqui
Original Papers
Received:

Summary

Human skin allografts cultured for approximately 6 weeks on a solid medium were transplanted to non-related human recipients with skin defects of various etiology. Although based on this study no answer could be given to the question whether these grafts survived or were replaced by cells of the recipient, it was observed that in most cases the grafts were not rejected and remained in situ several months up to over a year.

Key words

Allograftculture Leg ulcer Re-epithelialization 

Zusammenfassung

Haut-Allograftkulturen (Vollhaut, subcutanes Gewebe entfernt oder dicke Dermatomschnitte) die 6 Wochen lang auf Festkulturen gezüchtet wurden, wurden auf Patienten mit Hautdefekten verschiedener Ursache transplantiert. Diese Transplantate wurden nicht abgestoßen und blieben für mehrere Monate bis 1 Jahr in situ lebensfähig. Die Frage, ob diese Transplantate überleben oder durch Empfängerzellen ersetzt wurden, wird diskutiert.

Schlüsselwörter

Allograft-Kultur Ulcera cruris Reepithelisation 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Koumans, R. K. J., Burke, J. F.: Skin allografts and immunosuppression in the treatment of massive thermal injury. Surgery 66, 89–96 (1969)Google Scholar
  2. 2.
    Koch, C. T., Frederiks, E., Eysvoogel, V. P., Rood, J. van: Mixed-lymphocyte-culture and skin-grafts data in unrelated HL-A identical individuals. The Lancet 1971II, 1334–1336Google Scholar
  3. 3.
    Hardin, C. A., Werder, A. A.: The effect of total body irradiation on the survival of homologous skin grafts on CFW mice. Plast. Reconstruct. Surg. 13, 40 (1954)Google Scholar
  4. 4.
    Levinson, M. E., Necheles, H.: Successful prolongation of survival of skin homografts. Plast. Reconstruct. Surg. 17, 218 (1956)Google Scholar
  5. 5.
    Hume, D. M., Nelson, D. H., Miller, D. W.: Blood and urinary 17-hydroxysteroids in patients with severe burns. Ann. Surg. 143, 316 (1956)Google Scholar
  6. 6.
    Evans, E. I., Butterfield, W. J. H.: The stress response in the severely burned. Ann. Surg. 134, 588 (1951)Google Scholar
  7. 7.
    Billingham, R. E., Krohn, P. L., Medawar, P. B.: Effect of locally applied cortisone acetate on survival of skin homografts in rabbits. Br. Med. J. 3, 1049 (1951)Google Scholar
  8. 8.
    Billingham, R. E., Boswell, T.: Studies on the problem of corneal homografts. Proc. Roy. Soc. London 141, 392 (1953)Google Scholar
  9. 9.
    Klaue, P.: Erfolgreiche Vorbehandlung von Kaninchen-Hautallotransplantaten mit Triamcinolonazetonid. Brun's Beitr. Klin. Chir. 221, 4, 313–318 (1974)Google Scholar
  10. 10.
    Weisman, P., Cannon, B.: The failure of heat treatment to prolong the survival of skin homografts in guinea pigs: a pilot experiments. Plast. Reconstruct. Surg. 8, 428 (1951)Google Scholar
  11. 11.
    Baxter, H. Entin, M. A.: Clinical study of the fate of homografts in man: effects of repeated dosage of the same donor and refrigeration on these grafts. Am. J. Surg. 81, 285 (1951)Google Scholar
  12. 12.
    Sewell, W. H., Koth, D. R., Pate, J. W., Bedell, W. C.: Review of some experiments with freeze-dried grafts. Am. J. Surg. 91, 358 (1956)Google Scholar
  13. 13.
    Hardin, C. A., Werder, A. A.: Effect of skin extracts on the viability of homologous skin grafts in mice. Ann. New York Acad. Sci. 59, 381 (1955)Google Scholar
  14. 14.
    Felton, L. D.: The significance of antigen in animal tissues. J. Immunol. 61, 107 (1949)Google Scholar
  15. 15.
    Morgan, P., Watson, D. W., Cromartie, W. J.: Type specificity of immunological paralysis induced in mice with pneumococcal type II polysaccharide. J. Bact. 65, 224 (1953)Google Scholar
  16. 16.
    Stark, R. B., Conway, H., Sedar, J. D.: Observations on the circulation of homologous grafts of skin recipients treated with sodium salicylate, histadyl and irradiation. Ann. New York Acad. Sci. 59, 343 (1955)Google Scholar
  17. 17.
    Siddiqui, A. H., Janssen de Limpens, A. M. P., Cormane, R. H.: In vitro organ culture of adult human and mouse skin. Acta Derm. Venereol. 54, 255–258 (1974)Google Scholar
  18. 18.
    Janssen de Limpens, A. M. P., Siddiqui, A. H., Cormane, R. H.: Preliminary studies on the prolongation of the survival of mouse skin allografts cultured on a solid demium. Arch. Derm. Forsch. 252, 187–192 (1975)Google Scholar
  19. 19.
    Tio, D., Siddiqui, A. H.: The fate of cultured human skin allografts applied to chronic leg ulcers (in preparation)Google Scholar
  20. 20.
    Difco Manual of Dehydrated Culture Media and Reagent for Microbiological and Clinical Laboratory Procedures. Detroit, Michigan: Difco Labs. 1971Google Scholar
  21. 21.
    Toolan, H. W.: Continued growth of various embryo homografts in cortisone-treated adults and of young embryo skin in non-treated hosts. Transpl. Bull. 4, 107 (1957)Google Scholar
  22. 22.
    Treensegaard-Hansen, E.: Amnion implantation in peripheral vascular disease. Br. Med. J. 2, 262 (1956)Google Scholar
  23. 23.
    Siddiqui, A. H., Janssen de Limpens, A. M. P., Cormane, R. H.: Specific y-chromosome fluorescence in interphase nuclei of epithelial cells in human buccal smears. Arch. Derm. Res. (in press)Google Scholar
  24. 24.
    Pear, L. A.: Cell survival theory versus replacement theory. Plast. Reconstruct. Surg. 16, 161 (1955)Google Scholar
  25. 25.
    Rowden, G., Lewis, M. G., Summerlin, W. T.: An EM-immunoenzyme investigation on human skin tissue culture (Abstract). Proc. Ist Ann. Canad. Microsc. Soc. 1974Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • A. M. P. Janssen de Limpens
    • 1
  • R. H. Cormane
    • 1
  • A. H. Siddiqui
    • 1
  1. 1.Department of DermatologyUniversity of AmsterdamAmsterdamThe Netherlands

Personalised recommendations