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Growth of Escherichia coli B/r/l in a semi-continuous system designed for the synchronization of cell division

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Abstract

Escherichia coli B/r/l was grown under conditions of periodic feeding. Glucose, the only carbon source, was supplied at intervals longer than the generation time of the organism. Thus, each period of glucose availability was followed by a period of depletion. This process gave rise to two synchronous populations, one dividing shortly after a new supply of fresh medium and the other dividing at a later stage within the feeding cycle. Thymine incorporation experiments suggested that the double population emerged as the result of a discriminatory blockage of DNA replication.

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References

  1. Anagnostopoulos, G. D.: Unbalanced growth in a semicontinuous culture system designed for the synchronization of cell division. J. gen. Microbiol. 65, 23–33 (1971)

  2. Anagnostopoulos, G. D.: Defining synchrony of cell cultures. Arch. Microbiol. 104, 95–96 (1975)

  3. Bonhoeffer, F., Gierer, A.: On the growth mechanism of the bacterial chromosome. J. molec. Biol. 70, 534–540 (1963)

  4. Dawson, P. S. S.: Continuous phased growth with a modified chemostat. Canad. J. Microbiol. 11, 893–903 (1965)

  5. Nishi, A., Okamura, S., Yanagita, T.: Shift of cell-age distribution patterns in later phases of E. coli culture. J. gen. appl. Microbiol. 13, 103–119 (1967)

  6. Pirt, S. J.: A kinetic study of the mode of growth of surface colonies of bacteria and fungi. J. gen. Microbiol. 47, 181–197 (1967)

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Buckley, D.E., Anagnostopoulos, G.D. Growth of Escherichia coli B/r/l in a semi-continuous system designed for the synchronization of cell division. Arch. Microbiol. 105, 169–172 (1975). https://doi.org/10.1007/BF00447132

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Key words

  • Synchronization
  • Induction
  • Periodic Feeding