Archives of Microbiology

, Volume 142, Issue 2, pp 190–193 | Cite as

Evidence for cAMP-mediated control of isoleucyl-tRNA synthetase formation in Escherichia coli K-12

  • Arthur L. Williams
  • Richard S. Barnett
Original Papers


The ability of cAMP to inhibit isoleucyl-tRNA synthetase (IRS) formation has been demonstrated in wild type K-12 Escherichia coli and two adenyl-cyclase (cya) mutants. cAMP appeared not to have any effect on either the valyl- or arginyl-tRNA synthetase (VRS and ARS respectively). Addition of cAMP led to a reduction in rate of IRS synthesis but not VRS or ARS. Furthermore, derepression of IRS and VRS by isoleucine limitation was completely prevented by cAMP.

Key words

cAMP Isoleucyl-tRNA synthetase Regulation Gene Escherichia coli Expression 



isoleucyl-tRNA synthetase


valyl-tRNA synthetase


arginyl-tRNA synthetase


cyclic adenosine-3′,5′-monophosphate


adenyl cyclase Gene


cAMP receptor protein


optical density


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Arthur L. Williams
    • 1
  • Richard S. Barnett
    • 1
  1. 1.School of Biological SciencesUniversity of KentuckyLexingtonUSA

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