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Archives of Microbiology

, Volume 142, Issue 2, pp 190–193 | Cite as

Evidence for cAMP-mediated control of isoleucyl-tRNA synthetase formation in Escherichia coli K-12

  • Arthur L. Williams
  • Richard S. Barnett
Original Papers

Abstract

The ability of cAMP to inhibit isoleucyl-tRNA synthetase (IRS) formation has been demonstrated in wild type K-12 Escherichia coli and two adenyl-cyclase (cya) mutants. cAMP appeared not to have any effect on either the valyl- or arginyl-tRNA synthetase (VRS and ARS respectively). Addition of cAMP led to a reduction in rate of IRS synthesis but not VRS or ARS. Furthermore, derepression of IRS and VRS by isoleucine limitation was completely prevented by cAMP.

Key words

cAMP Isoleucyl-tRNA synthetase Regulation Gene Escherichia coli Expression 

Abbreviations

IRS

isoleucyl-tRNA synthetase

VRS

valyl-tRNA synthetase

ARS

arginyl-tRNA synthetase

cAMP

cyclic adenosine-3′,5′-monophosphate

Cya

adenyl cyclase Gene

CRP

cAMP receptor protein

O.D.

optical density

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Arthur L. Williams
    • 1
  • Richard S. Barnett
    • 1
  1. 1.School of Biological SciencesUniversity of KentuckyLexingtonUSA

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